Overview | AllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction. TheChIPAb+p53setincludesthep53antibodyraisedagainstrecombinanthumanp53,thenegativecontrolantibody(mousenormalIgG),andqPCRprimersflankinganSp1bindingsite(towhichp53canberecruited1)withinthehumanp21(WAF1/CIP1/CDKN1A)promoter,amplifyinga105basepairPCRproduct.Thep53andnegativecontrolantibodiesaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofp53associatedchromatin. |