Overview | AllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction. TheChIPAb+HDAC1setincludestheHDAC1antibody,thenegativecontrolantibodysupernatant(mouseIgG),andqPCRprimersflankinganSp1bindingsiteinhumanp21(WAF1/CIP1/CDKN1A)promoter,amplifyinga105basepairPCRproduct.TheHDAC1andnegativecontrolantibodiesaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofHDAC1associatedchromatin. |