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Millipore/17-600 | ChIPAb+ CREB - ChIP Validated Antibody and Primer Set, rabbit monoclonal/17-600/25 assays25 assays pe
  • Millipore/17-600 | ChIPAb+ CREB - ChIP Validated Antibody and Primer Set, rabbit monoclonal/17-600/25 assays25 assays pe

Millipore/17-600 | ChIPAb+ CREB - ChIP Validated Antibody and Primer Set, rabbit monoclonal/17-600/25 assays25 assays pe

价格: ¥4800.00 市场价: 8000.00
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货号: 17-600
品牌: Millipore
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    • Description
      CatalogueNumber17-600
      BrandFamilyUpstate
      TradeName
      • ChIPAb+
      • Upstate
      DescriptionChIPAb+CREB-ChIPValidatedAntibodyandPrimerSet,rabbitmonoclonal
      OverviewEverylotoftheChIPAb+lineofantibodiesisindividuallyvalidatedforchromatinprecipitation,inordertoguaranteesuccessfulChIPassayseverytime.Eachantibodyincludesacontrolprimersetforperformanceconfirmation.CREBantibodyandthenegativecontrolantibody(rabbitnormalIgG)canbeusedtodemonstratethattheCREBantibodyisfunctionallyvalidatedintheprecipitationofCREBassociatedchromatin.
      TheqPCRprimersincludedflanktheCREBbindingsiteinhumanNR4A2promoter.
      AlternateNames
      • activetranscriptionfactorCREB
      • cAMPresponsiveelementbindingprotein1
      • cAMP-responseelement-bindingprotein-1
      • cAMP-responsiveelement-bindingprotein1
      • transactivatorprotein
      BackgroundInformationAllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction.
      TheChIPAb+CREB,antibody/primersetincludestheCREB,antibody,anegativecontrolantibody(purifiedrabbitIgG),andqPCRprimerswhichamplifya64bpregionflankingtheCREofthehumancFosgene.TheCREBandnegativecontrolculturedsupernatantaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofCREBassociatedchromatin.
      ProductInformation
      Components
      • Anti-CREB(RabbitmonoclonalIgG):PartNo.CS203204.Onevialcontaining250μLofculturedsupernatantcontaining0.05%sodiumazide.Storeat-20°C.
        NegativeControlSupernatant:PartNo.CS203202.Onevialcontaining250μLofculturedsupernatantcontaining0.05%sodiumazide.Storeat-20°C.
        ChIPPrimerscFosCRE:PartNo.CS203203.Onevialcontaining75μLof5μMofeachprimerspecificforthecFosCRE.Storeat-20°C.
        FOR:GGCCCACGAGACCTCTGAGACA
        REV:GCCTTGGCGCGTGTCCTAATCT
      FormatCultureSupernatant
      Control
      • IncludesnegativecontrolculturedsupernatantandprimersspecificforofhumancFosCRE.
      Presentation25assaysperset.Recommendeduse:~10μLantibodyperchromatinimmunoprecipitation(dependentuponbiologicalcontext).
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
      Applications
      ApplicationThisChIPAb+CREB-ChIPValidatedAntibody&PrimerSetconvenientlyincludestheantibody&thespecificcontrolPCRprimers.
      KeyApplications
      • WesternBlotting
      • ChromatinImmunoprecipitation(ChIP)
      ApplicationNotesChromatinImmunoprecipitation:
      Representativelotdata.
      Sonicatedchromatinpreparedfrom293Tcells(5X106cellequivalentsperIP)wassubjectedtochromatinimmunoprecipitationusing10µLofeitheranegativesupernatant,orCREBantibodyandtheMagnaChIP™AKit(Cat.#17-610).
      SuccessfulimmunoprecipitationofCREB-associatedDNAfragmentswasverifiedbyqPCRusingcFosdownstream4Kb(negative)andcFosCRE(positive)(Figure2).DataispresentedaspercentinputofeachIPsamplerelativetoinputchromatinforeachampliconandChIPsampleasindicated.
      PleaserefertotheEZ-MagnaAChIP™(Cat.#17-408)orEZ-ChIP™(Cat.#17-371)protocolforexperimentaldetails.

      WesternBlotAnalysis:
      Representativelotdata.
      HeLanuclearextractwasresolvedbyelectrophoresis,transferredtonitrocelluloseandprobedwithanti-CREB,cloneNL904(1:8,000dilution)
      Proteinswerevisualizedusingagoatanti-rabbitsecondaryantibodyconjugatedtoHRPandachemiluminescencedetectionsystem.
      ArrowindicatesCREB(~43kDa).
      BiologicalInformation
      ImmunogenCREBpurifiedantibodyismadeagainstaKLH-conjugatedsyntheticpeptidecorre-spondingtoaminoacids5-24ofhumanCREB.
      Epitopea.a.5-24
      CloneNL904
      HostRabbit
      SpecificityRecognizesCREB,MW~43kDa.
      IsotypeIgG
      SpeciesReactivity
      • Human
      • Mouse
      • Rat
      SpeciesReactivityNoteHuman,mouse,rat.Nottestedinotherspecies.Theimmunogensequenceisidenticalinawiderangeofanimalandplantspecies,sobroadcross-reactivityisexpected.
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesatranscriptionfactorthatisamemberoftheleucinezipperfamilyofDNAbindingproteins.ThisproteinbindsasahomodimertothecAMP-responsiveelement,anoctamericpalindrome.Theproteinisphosphorylatedbyseveralproteinkinases,andinducestranscriptionofgenesinresponsetohormonalstimulationofthecAMPpathway.Alternatesplicingofthisgeneresultsintwotranscriptvariantsencodingdifferentisoforms.
      GeneSymbol
      • CREB1
      • MGC9284
      • CREB
      UniProtNumber
      UniProtSummaryFUNCTION:ThisproteinbindsthecAMPresponseelement(CRE),asequencepresentinmanyviralandcellularpromoters.CREBstimulatestranscriptiononbindingtotheCRE.TransciptionactivationisenhancedbytheTORCcoactivatorswhichactindependentlyofSer-133phosphorylation.ImplicatedinsynchronizationofcircADIanrhythmicity.
      SUBUNITSTRUCTURE:InteractswithPPRC1.BindsDNAasadimer.ThisdimerisstABIlizedbymagnesiumions.Interacts,throughthebZIPdomain,withthecoactivatorsTORC1/CRTC1,TORC2/CRTC2andTORC3/CRTC3.WhenphosphorylatedonSer-133,bindsCREBBPBysimilarity.
      SUBCELLULARLOCATION:Nucleus.
      PTM:Stimulatedbyphosphorylation.PhosphorylationofbothSer-133andSer-142intheSCNregulatestheactivityofCREBandparticipatesincircadianrhythmgeneration.PhosphorylationofSer-133allowsCREBBPbindingBysimilarity.PhosphorylateduponDNAdamage,probablybyATMorATR.
      INVOLVEMENTINDISEASE:AchromosomalaberrationinvolvingCREB1isassociatedwithangiomatoidfibroushistiocytoma(AFH)[MIM:612160].Translocationt(2;22)(q33;q12)withCREB1generatesaEWSR1/CREB1fusiongenethatismostcommongeneticabnormalityinthistumortype.
      SEQUENCESIMILARITIES:BelongstothebZIPfamily.Contains1bZIPdomain.Contains1KID(kinase-inducIBLe)domain.
      MolecularWeight~43kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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