当前位置 ： Millipore >>> Millipore/17-478 | phospho-PRAS40 (Thr246) STAR ELISA Kit/17-478/96 assays
Millipore/17-478 | phospho-PRAS40 (Thr246) STAR ELISA Kit/17-478/96 assays
  • Millipore/17-478 | phospho-PRAS40 (Thr246) STAR ELISA Kit/17-478/96 assays

Millipore/17-478 | phospho-PRAS40 (Thr246) STAR ELISA Kit/17-478/96 assays

价格: 试用 市场价: 0.00
打印产品信息
货号: 17-478
品牌: Millipore
规格
数量
库存(0)
特别 提示
代购产品：无质量问题不接受退换货，下单前请仔细核对信息。
下单后请及时联系客服核对商品价格，订单生效后再付款。
立即申请

收藏商品
资深产品顾问
咨询顾问

全国免费服务热线

4000-520-616

  • 自营商城 一站式服务
  • 厂家直采 剔除溢价
  • 品质甄选 正品保证
  • 严控流程 只做188精品
  • 极速物流 如约送货
  • 详情
  • 使用说明
  • 常见问题
    • Description
      CatalogueNumber17-478
      BrandFamilyUpstate
      TradeName
      • STAR
      • Upstate
      Descriptionphospho-PRAS40(Thr246)STARELISAKit
      AlternateNames
      • SignalTranscductionAssayReactionPRAS40ELISA
      BackgroundInformationI.TESTPRINCIPLE
      TheUPSTATE®colorimetricSTAR(SignalTransductionAssayReaction)ELISAkitisasolidphasesandwichenzymelinkedimmunosorbentassaythatprovidesafast,sensitivemethodtodetectspecificlevelsofsignalingtargetsinwholecellextracts.ThePRAS40plateiscoatedwithaspecificmousemonoclonalPRAS40captureantibodyonthemicrowellsofthe96-wellclearplate.SamplelysateorthestandardincludedinthekitareincubatedinthemicrowellsallowingPRAS40antigentobecapturedintheplatewells.Theplateisthenwashedtoremoveanyunboundnon-specificmaterial.Thewellsarethenincubatedwithaspecificrabbitanti-phospho-PRAS40(Thr246)antibodytodetectthecapturedPRAS40ontheplatewellthatisphosphorylatedonThr246.TheunbounddetectionantibodyiswashedawayfollowedbyincubationwithanHRP-conjugatedanti-rabbitantibody.AftertheadditionofTMBsubstrateandstopsolutiontheabsorbanceismeasuredat450nmusingaplatereader.Thisallowsforasensitiveenzymaticdetectionofthesample.
      Theentireassaytakeslessthan5hourstocompletewithminimalhands-ontime.Manyofthereagentsaresuppliedinready-touseformulationsforeaseofuse.ThekitalsoincludesastandardthatisrunasbothapositivecontrolandtogenerateastandardcurveforphosphorylatedPRAS40(Thr246)measurement.

      II.PRAS40BACKGROUND
      PRAS40(ProlineRichAktSubstrate,40kDa),alsoknownasAKT1S1,isaubiquitouslyexpressproteinthatisphosphorylatedonresidueThr246viathePI3K/Aktpathway.ItwasoriginallydiscoveredasanAktsubstrateasithastheputativeAktphosphorylationmotifofRxRxxpS/pT2.Thisphosphorylationallowsittobind14-3-3andRaptorofthemTORcomplexmTORC1.PRAS40isknowntobindtoandregulatemTORC1(mTOR,Raptor,mLST8)kinasesactivitythatisactivatedbyinsulindownstreamofPI3KandAktandsubsequentlyphosphorylatesp70S6Kand4EBP1.PRAS40isknowntohaveaputativeTOSmotif(FVMDE)thatallowsitbindtoRaptorofmTORC1intheabsenceofinsulin1.ItisthoughtthatthroughitbindingtotheRaptor,PRAS40inhibitsthekinaseactivityofmTORC1bypreventingtobindtoitssubstratessuchasp70S6Kand4EBP1.
      MaterialsRequiredbutNotDelivered1.Multi-channelorrepeatingPipettes
      2.Plateshaker(optional)
      3.Pipettorsandtipscapableofaccuratelymeasuring1-1000µL
      4.GraduatedSEROlogicalpipettes
      5.96-wellmicrotiterPlateReaderwith450nmfilter
      6.Graphingsoftwareforplottingdataorgraphpaperformanualplottingofdata
      7.Microfugetubesforstandardandsampledilutions
      8.Mechanicalvortex
      9.1litercontainer
      10.Distilledordeionizedwater
      ProductInformation
      Components
      • 1.CapturePlatepre-coatedwithanti-PRAS40antibody:(PartNo.17-478A)Onepre-coated96-stripwellimmunoplatesealedinafoilpouch.
      • 2.Anti-phospho-PRAS40(Thr246)detectionantibody:(PartNo.17-478B)Onebottle(11mL)ofanti-phospho-PRAS40(Thr246)detectionantibodycontainingsodiumazide,readytouse.
      • 3.ELISADiluent:(PartNo.17-478C)Onebottle(25mL)ofELISADiluentcontainingsodiumazide,readytouse.
      • 5.Anti-RabbitIgGHRPconjugate:(PartNo.17-478E)Onevial(125μL)of100Xanti-rabbitHRPconjugate.
      • 6.HRPDiluent:(PartNo.17-478F)Onebottle(25mL)ofHRPDiluent.
      • 7.TMBSolution:(PartNo.17-478G)Onebottle(25mL)ofstABIlizedtetramethylbenzidine(TMB),readytouse
      • 8.StopSolution:(PartNo.17-478H)Onebottle(25mL)ofstopsolution,readytouse.
      • 9.Phospho-PRAS40(Thr246)Standard:(PartNo.17-478I)TwovialsofphosphorylatedPRAS40(Thr246)standard,lyophilized.
      • 10.PlateCovers:Twoplatecovers.
      DetectionmethodColorimetric
      StorageandShippingInformation
      StorageConditionsMaintaintheunopenedkitat2-8°Cuntilexpirationdate.
      Precautions
      •Theinstructionsprovidedhavebeendesignedtooptimizethekit"sperformance.Deviationfromtheinstructionsmayresultinsuboptimalperformanceofthekitandthefailuretoproduceaccuratedata.
      •CausticMaterial:StopSolution.Caution:Eye,hand,face,andclothingprotectionshouldbewornwhenhandlingthismaterial.
      •SafetyWarningsandPrecautions:Thiskitisdesignedforresearchuseonlyandnotrecommendedforinternaluseinhumansoranimals.Allchemicalsshouldbeconsideredpotentiallyhazardousandprinciplesofgoodlaboratorypracticeshouldbefollowed.
      •TheDetectionAntibodyandELISADiluentcontainsodiumazide.Sodiumazidemayreactwithcopperandleadplumbingtoformhighlyexplosivemetalazides.Upondisposal,flushwithlargeamountsofwatertopreventazidebuild-up.Avoidcontactwithskin.
      •TheAnti-RabbitIgGHRPConjugateandHRPDiluentcontainthimerosal.Thimerosalishighlytoxicbyinhalation,contactwithskinorifswallowed.ThimerosalisapossIBLemutagenandshouldbehandledaccordingly.
      Applications
      ApplicationThephospho-PRAS40(Thr246)colorimetricSTARELISAkitisasolidphasesandwichenzymelinkedimmunosorbentassaythatprovidesafast,sensitivemethodtoquantifyspecificlevelsofsignalingtargetsindenaturedcellextracts.
      KeyApplications
      • ELISA
      BIOLOGicalInformation
      SpeciesReactivity
      • Human
      AnalytesAvailable
      • phospho-PRAS40(Thr246)
      EntrezGeneNumber
      EntrezGeneSummaryAKT1S1isaproline-richsubstrateofAKT(MIM164730)thatbinds14-3-3protein(seeYWHAH,MIM113508)whenphosphorylated(Kovacinaetal.,2003[PubMed12524439]).[suppliedbyOMIM]
      GeneSymbol
      • AKT1S1
      • Lobe
      • MGC2865
      • PRAS40
      Modifications
      • Phosphorylation
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:Q96B36#Mayplayanimportantroleinphosphatidylinositol3-kinase(PI3K)-AKT1survivalsignaling.SubstrateforAKT1phosphorylation,butcanalsobeactivatedbyAKT1-independentmechanisms.Itsroleinsurvivalsignalingpathwaysmaybemodulatedbyoxidativestress.Mayalsoplayaroleinnervegrowthfactor-mediatedneuroprotection.
      SIZE:256aminoacids;27383Da
      SUBUNIT:Thephosphorylatedforminteractswith14-3-3.
      SUBCELLULARLOCATION:Cytoplasm,cytosol(Bysimilarity).Note=Foundinthecytosolicfractionofthebrain(Bysimilarity).
      TISSUESPECIFICITY:Widelyexpressedwithhighestlevelsofexpressioninliverandheart.Expressedathigherlevelsincancercelllines(e.g.A549andHeLa)thaninnormalcelllines(e.g.HEK293).
      PhysicochemicalInformation
      Sensitivity
      • Sensitivity:1unit/mL
        RangeofDetection:1.6to100units/mL
        SpeciesReactivity:Human
      Dimensions
      MaterialsInformation
      MaterialsInformation
    售后保障
    蚂蚁淘生物188，秉承蚂蚁淘一贯的严谨态度，由蚂蚁淘公司专业人员负责品控、采购、物流、销售、售后，保障正品优质。以“快速好省，为科研提供好产品、好价格”为理念，直接链接原厂家，从全国各地原制造商严格挑选188款科研精品，剔除品牌溢价，188生物新电商，把好的产品带给科研！  力求给你最优质的商品。
  • Q：生物188产品正品保障吗？
    A：生物188质量把控人员具有十年的从业经验，在业界享有良好的口碑；自营商城，直接从厂家采购， 自己的团队负责国际物流和清关，中间没有第三方，所有流程严格把控，100%保证正品，假一罚十。

    Q：下单后可以修改订单吗？
    A：下单后的商品付款之前可以修改；订单付款成功，需要联系我们客服进行修改；客服电话：4000-520-616

    Q：可以开发票吗？
    A：本网站所售商品都是正规清关，均开具16%正规发票，发票金额含配送费金额，另有说明的除外。

    Q：商品几天可以发货？
    A：生物188商品，全部现货销售，付款后即可发货,一般一周内送达！

    Q：如何联系商家？
    A：有任何问题够可以电话咨询我们，全国24小时免费服务热线：4000-520-616 或联系我们的在线客服QQ：1570468124

    Q：收到的商品少了/发错了怎么办？
    A：同个订单购买多个商品可能会分为一个以上包裹发出，可能不会同时送达，建议查看订单详情是否是 部分发货状态；如未收到，可联系在线客服或者致电4000-520-616。

    Q：退换货/维修需要多长时间？
    A：一般情况下，退货处理周期为客户收到产品一个月内（以快递公司显示签收时间为准），包装规格、 数量、品种不符，外观毁损、短缺或缺陷，请在收到货24小时内申请退换货；特殊商品以合同条款为准。

客户服务

在线客服
用户反馈

何为188

极简而严谨，我们仅销售188款生物医学科研用品，款款都是爆款；因为少所以聚焦，聚焦甄选每一款产品，聚焦服务每一位客户!

关注我们 :

点击QQ联系我们
生物188微信

关注188微信公众号

获取最新优惠活动通知

  • 品质甄选，正品保证

  • 自营电商，厂家直采

  • 极简主义，188精品

常见问题 | 购买相关 | 配送与验收 | 支付问题 | 售后与服务 | 服务协议 | 隐私政策 | 何为188 | 中国智造 | 联系我们
友情链接： Adventbio中国 | Polysciences第二代聚合物转染 | IHCWORLD公司 | 华东地区代理echelon | AsiaMercury1产品代理 | dextra厂家 | Lumigen Inc代理 | 蚂蚁淘 | 广州驭时医药科技有限公司

公司版权所有 © 2005-2021 苏州蚂蚁淘生物科技有限公司 苏ICP备17049038号-10