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Millipore/17-474 | STAR phospho-MEK1 (Ser218/222) ELISA Kit/17-474/96 assays
  • Millipore/17-474 | STAR phospho-MEK1 (Ser218/222) ELISA Kit/17-474/96 assays

Millipore/17-474 | STAR phospho-MEK1 (Ser218/222) ELISA Kit/17-474/96 assays

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货号: 17-474
品牌: Millipore
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    • Description
      CatalogueNumber17-474
      BrandFamilyUpstate
      TradeName
      • STAR
      • Upstate
      DescriptionSTARphospho-MEK1(Ser218/222)ELISAKit
      BackgroundInformationTheUPSTATEcolorimetricSTAR(SignalTransductionAssayReaction)ELISAkitisasolidphasesandwichenzymelinkedimmunosorbentassaythatprovidesafast,sensitivemethodtodetectspecificlevelsofsignalingtargetsindenaturedcellextracts.TheMEK1plateiscoatedwithaspecificmousemonoclonalanti-MEK1captureantibodyonthemicrowellsofthe96-wellclearplate.SamplelysateorthestandardincludedinthekitareincubatedinthemicrowellsallowingMEK1antigentobecapturedintheplatewells.Theplateisthenwashedtoremoveanynon-boundunspecificmaterial.Thewellsarethenincubatedwithaspecificrabbitanti-phospho-MEK1(Ser218/222)antibodytodetectthecapturedontheplatewell.TheunbounddetectionantibodyiswashedawayfollowedbyincubationwithanHRP-conjugatedanti-rabbitantibody.Thisallowsforasensitiveenzymaticdetectionofthesample.AftertheadditionofTMBsubstrateandstopsolutiontheabsorbanceismeasuredat450nmusingaplatereader.Theentireassaytakeslessthan5hourstocompletewithminimalhands-ontime.Manyofthereagentsaresuppliedinready-touseformulationsforeaseofuse.Thekitalsoincludesastandardthatisrunasbothapositivecontrolandtodevelopastandardcurve.
      MaterialsRequiredbutNotDelivered1.Multi-channelorrepeatingPipettes
      2.Plateshaker(optional)
      3.Pipettors&tipscapableofaccuratelymeasuring1-1000%micro;L
      4.GraduatedSEROlogicalpipettes
      5.96-wellmicrotiterPlateReaderwith450nmfilter
      6.Graphingsoftwareforplottingdataorgraphpaperformanualplottingofdata
      7.Microfugetubesforstandardandsampledilutions
      8.Mechanicalvortex
      9.1litercontainer
      10.Distilledordeionizedwater
      ProductInformation
      Components
      • 1.CapturePlatepre-coatedwithanti-MEK1antibody:(PartNo.17-474A)Onepre-coated96-stripwellimmunoplatesealedinafoilpouch.
      • 2.Anti-phospho-MEK1(Ser218/222)detectionantibody:(PartNo.17-474B)Onebottle(11mL)ofanti-phospho-MEK1(Ser218/222)detectionantibodycontainingsodiumazide,readytouse.
      • 3.ELISADiluent:(PartNo.17-474C)Onebottle(25mL)ofELISADiluentcontainingsodiumazide,readytouse.
      • 4.25XELISAWashBuffer:(PartNo.17-474D)Onebottle(50mL)of25XELISAWashBuffer.
      • 5.Anti-RabbitIgGHRPconjugate:(PartNo.17-474E)Onevial(125µL)of100Xanti-rabbitHRPconjugatecontainingthimerosol.
      • 6.HRPDiluent:(PartNo.17-474F)Onebottle(25mL)ofHRPDiluentcontainingthimerosol.
      • 7.TMBSolution:(PartNo.17-474G)Onebottle(25mL)ofstABIlizedtetramethylbenzidine(TMB),readytouse.
      • 8.StopSolution:(PartNo.17-474H)Onebottle(25mL)ofstopsolution,readytouse.
      • 9.Phospho-MEK1(Ser218/222Standard:(PartNo.17-474I)Twovialsofphospho-MEK1(Ser218/222)standard,lyophilized.
      • 10.SampleIncubationBuffer:(PartNo.17-73J)Onebottle(10mL)ofasampleincubation(denaturing)buffer,readytouse.
      • 11.PlateCovers:Twoplatecovers.
      DetectionmethodChromogenic
      StorageandShippingInformation
      StorageConditions2yearsat4°C
      Applications
      KeyApplications
      • ELISA
      BIOLOGicalInformation
      SpeciesReactivity
      • Human
      • Mouse
      AnalytesAvailable
      • MEK1
      EntrezGeneNumber
      EntrezGeneSummaryTheproteinencodedbythisgeneisamemberofthedualspecificityproteinkinasefamily,whichactsasamitogen-activatedprotein(MAP)kinasekinase.MAPkinases,alsoknownasextracellularsignal-regulatedkinases(ERKs),actasanintegrationpointformultiplebiochemicalsignals.ThisproteinkinaseliesupstreamofMAPkinasesandstimulatestheenzymaticactivityofMAPkinasesuponwidevarietyofextra-andintracellularsignals.AsanessentialcomponentofMAPkinasesignaltransductionpathway,thiskinaseisinvolvedinmanycellularprocessessuchasproliferation,differentiation,transcriptionregulationanddevelopment.
      GeneSymbol
      • MAP2K1
      • MAPKK1
      • PRKMK1
      • MEK1
      • MKK1
      Modifications
      • Phosphorylation
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:Q02750#CatalyzestheconcomitantphosphorylationofathreonineandatyrosineresidueinaThr-Glu-TyrsequencelocatedinMAPkinases.ActivatesERK1andERK2MAPkinases.
      SIZE:393aminoacids;43439Da
      SUBUNIT:InteractswithMORG1(Bysimilarity).InteractswithYersiniayopJ.
      PTM:PhosphorylationonSer/ThrbyMAPkinasekinasekinases(RAForMEKK1)regulatespositivelythekinaseactivity.&AcetylationbyYersiniayopJpreventsphosphorylationandactivation,thusblockingtheMAPKsignalingpathway.
      DISEASE:SwissProt:Q02750#DefectsinMAP2K1areacauseofcardiofaciocutaneoussyndrome(CFCsyndrome)[MIM:115150];alsoknownascardio-facio-cutaneoussyndrome.CFCsyndromeischaracterizedbyadistinctivefacialappearance,heartdefectsandmentalretardation.Heartdefectsincludepulmonicstenosis,atrialseptaldefectsandhypertrophiccardiomyopathy.Someaffectedindividualspresentwithectodermalabnormalitiessuchassparse,friablehair,hyperkeratoticskinlesionsandageneralizedichthyosis-likecondition.TypicalfacialfeaturesaresimilartoNoonansyndrome.Theyincludehighforeheadwithbitemporalconstriction,hypoplasticsupraorbitalridges,downslantingpalpebralfissures,adepressednasalbridge,andposteriorlyangulatedearswithprominenthelices.TheinheritanceofCFCsyndromeisautosomaldominant.
      SIMILARITY:SwissProt:Q02750##Belongstotheproteinkinasesuperfamily.STESer/Thrproteinkinasefamily.MAPkinasekinasesubfamily.&Contains1proteinkinasedomain.
      PhysicochemicalInformation
      Sensitivity
      • Sensitivity:0.75Units/mL.
        RangeofDetection:1.6to100Units/mL
      Dimensions
      MaterialsInformation
      MaterialsInformation
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