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Millipore/17-320 | Histone Deacetylase Assay Kit (HDAC)/17-320/1 kitKit capacity: 100 assays
  • Millipore/17-320 | Histone Deacetylase Assay Kit (HDAC)/17-320/1 kitKit capacity: 100 assays

Millipore/17-320 | Histone Deacetylase Assay Kit (HDAC)/17-320/1 kitKit capacity: 100 assays

价格: ¥3948.00 市场价: 6580.00

货号: 17-320
品牌: Millipore
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    • Description
      CatalogueNumber17-320
      BrandFamilyUpstate
      TradeName
      • Upstate
      DescriptionHistoneDeacetylaseAssayKit(HDAC)
      OverviewThiskitcontainsnonrADIoactivecomponentsforradiolabelingandpurifyinghistoneH4peptidewith[3H]-acetylCoA.TheradiolabeledpeptideisasuitablesubstrateforassayofHDACactivity.HeLanuclearextract,whichisrichinhistonedeacetylaseactivity,isincludedasapositivecontrol.TheHDACinhibitorsodiumbutyrateisprovidedtodemonstratespecificityofthedeacetylaseactivity.
      ProductInformation
      Components
      • HeLaNuclearExtract(Cat.#12-309)
      • HistoneH4Peptide,biotinconjugate,residues2-24(Cat.#12-372)
      • HATAssayBuffer,5X(Cat.#20-148)
      • SodiumButyrate(Cat.#19-137)
      • 5XHDACAssayBuffer
      • StreptavidinAgarose
      • PCAFactive
      StorageandShippingInformation
      Applications
      ApplicationThiskitcontainsnonradioactivecomponentsforradiolabeling&purifyinghistoneH4peptidewith[3H]-acetylCoA.TheradiolabeledpeptideisasuitablesubstrateforassayofHDACactivity.
      KeyApplications
      • EnzymeAssays
      BIOLOGicalInformation
      EntrezGeneNumber
      EntrezGeneSummaryHistonesplayacriticalroleintranscriptionalregulation,cellcycleprogression,anddevelopmentalevents.Histoneacetylation/deacetylationalterschromosomestructureandaffectstranscriptionfactoraccesstoDNA.TheproteinencodedbythisgenebelongstotheclassIIhistonedeacetylase/acuc/aphafamily.Itpossesseshistonedeacetylaseactivityandrepressestranscriptionwhentetheredtoapromoter.ItcoimmunoprecipitatesonlywithHDAC3familymemberandmightformmulticomplexproteins.Italsointeractswithmyocyteenhancerfactor-2(MEF2)proteins,resultinginrepressionofMEF2-dependentgenes.Thisgeneisthoughttobeassociatedwithcoloncancer.Twotranscriptvariantsencodingdifferentisoformshavebeenfoundforthisgene.
      GeneSymbol
      • HDAC1
      • RPD3L1
      • GON-10
      • DKFZp686H12203
      • HD1
      • RPD3
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:Q9UQL6#ResponsIBLeforthedeacetylationoflysineresiduesontheN-terminalpartofthecorehistones(H2A,H2B,H3andH4).Histonedeacetylationgivesatagforepigeneticrepressionandplaysanimportantroleintranscriptionalregulation,cellcycleprogressionanddevelopmentalevents.Histonedeacetylasesactviatheformationoflargemultiproteincomplexes.InvolvedinmusclematurationbyrepressingtranscriptionofmyocyteenhancerMEF2C.Duringmuscledifferentiation,itshuttlesintothecytoplasm,allowingtheexpressionofmyocyteenhancerfactors.
      SIZE:1122aminoacids;121992Da
      SUBUNIT:InteractswithBCOR,HDAC7,HDAC9,CTBP1,MEF2C,NCOR2,NRIP1,PHB2anda14-3-3chaperoneprotein.InteractswithJARID1B.
      SUBCELLULARLOCATION:Nucleus.Cytoplasm.Note=Shuttlesbetweenthenucleusandthecytoplasm.Inmusclecells,itshuttlesintothecytoplasmduringmyocytedifferentiation.Theexporttocytoplasmdependsontheinteractionwitha14-3-3chaperoneproteinandisduetoitsphosphorylationatSer-259andSer-498byCaMK.TISSUESPECIFICITY:Ubiquitous.DOMAIN:SwissProt:Q9UQL6Thenuclearexportsequencemediatestheshuttlingbetweenthenucleusandthecytoplasm(Bysimilarity).
      PTM:PhosphorylatedbyCaMKatSer-259andSer-498.Thephosphorylationisrequiredfortheexporttothecytoplasm.&Ubiquitinated.Polyubiquitinationhoweverdoesnotleadtoitsdegradation.
      SIMILARITY:Belongstothehistonedeacetylasefamily.Type2subfamily.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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