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Millipore/17-10246 | ChIPAb+™ Histone H2A.Bbd - ChIP Validated Antibody and Primer Set/17-10246/25 assays25 assays per s
  • Millipore/17-10246 | ChIPAb+™ Histone H2A.Bbd - ChIP Validated Antibody and Primer Set/17-10246/25 assays25 assays per s

Millipore/17-10246 | ChIPAb+™ Histone H2A.Bbd - ChIP Validated Antibody and Primer Set/17-10246/25 assays25 assays per s

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货号: 17-10246
品牌: Millipore
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    • Description
      CatalogueNumber17-10246
      TradeName
      • ChIPAb+
      • Upstate
      DescriptionChIPAb+™HistoneH2A.BBD-ChIPValidatedAntibodyandPrimerSet
      OverviewAllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction.
      TheChIPAb+HistoneH2A.BbdsetincludestheHistoneH2A.Bbdantibody,aNormalRabbitIgG,andcontrolprimerswhichamplifya70bpregionofChIPprimers,PGK1.TheHistoneH2A.Bbdandnegativecontrolsaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofHistoneH2A.Bbd-associatedchromatin.
      AlternateNames
      • H2Ahistonefamily,memberB1
      • H2ABarrbody-deficient
      • histoneH2A-Bbdtype1
      BackgroundInformationHistoneH2A.Bbd(Barrbody-deficient),alsoknownashistoneH2A-Bbdtype1,isanatypicalhistonevariant.ThishistonevariantassociateswithH2BandreplaceshistoneH2Ainthenucleosome.IncontrasttootherH2Ahistones,thisvariantlacksconservedresiduesthataretargetsofpost-translationalmodifications.ConsequentlythisH2A.Bbdvariantisabout15aminoacidsshorterandonly48%similartothecanonicalH2A.ThemajordifferencesbetweenthetwoarefoundinthedockingdomainthatlinksH2A-H2BtotheH3-H4tetramer.Nucleosomescontainingthishistoneadoptamorerelaxedstructureandprotect~118basepairsofDNAinsteadofthe147basepairsfoundinclassicalnucleosomes.ThisvarianthistoneisassociatedwithactiveXchromosomesandautosomes,andistypicallyabsentfrominactiveXchromosomes.
      ProductInformation
      FormatAffinityPurified
      Control
      • IncludesnormalrabbitIgGandprimersspecificforhumanPGK1.
      PresentationAnti-HistoneH2A.Bbd(rabbitpolyclonal).Onevialcontaining100µgofpurifiedrabbitpolyclonalinbuffercontaining0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%sodiumazidebeforetheadditionof30%glycerol.Storeat-20°C
      Concentration:0.7mg/mL
      NormalRabbitIgG,.Onevialcontaining125µgofRabbitIgGin125µLofstoragebuffercontaining0.05%sodiumazide.Storeat-20°C.
      ChIPprimers,PGK1.Onevialcontaining75μLof5μMofeachprimerspecificforhumanPGK1.Storeat-20°C.
      FOR:CCTTCCCCCACGCTCCTA
      REV:TTGTGCAGGAACCTGTGAGATG
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
      Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.
      Applications
      ApplicationThisChIPAb+HistoneH2A.Bbd-ChIPValidatedAntibody&PrimerSetconvenientlyincludestheantibody&thespecificcontrolPCRprimers.
      KeyApplications
      • ElectrophoreticMobilityShiftAssay
      • Immunohistochemistry
      • Immunoprecipitation
      • WesternBlotting
      • ChromatinImmunoprecipitation(ChIP)
      ApplicationNotesWesternBlotAnalysis:
      Representativelotdata.
      H2A.Bbdoverexpression(Lane1-2),variousrecombinantHistoneproteins(Lane4-7)andHeLaacidextract(Lane8)lysateswereprobedwithAnti-HistoneH2A.Bbd(0.5µg/mL);
      Lane1:500ngofH2A.Bbdoverexpressionlysate
      Lane2:1µgH2A.Bbdoverexpressionlysate
      Lane3:500ngVectortransfectedcelllysatecontrol
      Lane4:500ngRecombinantHistoneH2A(Cat.#:14-493)
      Lane5:500ngRecombinantHistoneH2B(Cat.#:14-491)
      Lane6:500ngRecombinantHistoneH3(Cat.#:14-494)
      Lane7:500ngRecombinantHistoneH4(Cat.#:14-697)
      Lane8:10µgHeLaacidextract
      ProteinswerevisualizedusingaDonkeyAntiRabbitIgGsecondaryantibodyconjugatedtoHRPandachemiluminescencedetectionsystem.
      ArrowindicatesHistoneH2A.Bbd(~17kDa).(Figure2).
      BiologicalInformation
      ImmunogenKLH-conjugatedlinearpeptidecorrespondingtohumanHistoneH2A.Bbd.
      Epitopea.a.35-55
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostRabbit
      SpecificityThisantibodyrecognizeshumanHistoneH2A.Bbd.
      SpeciesReactivity
      • Human
      • Mouse
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryHistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Nucleosomesconsistofapproximately146bpofDNAwrappedaroundahistoneoctamercomposedofpairsofeachofthefourcorehistones(H2A,H2B,H3,andH4).Thechromatinfiberisfurthercompactedthroughtheinteractionofalinkerhistone,H1,withtheDNAbetweenthenucleosomestoformhigherorderchromatinstructures.ThisgeneencodesamemberofthehistoneH2Afamily.ThisgeneispartofaregionthatisrepeatedthreetimesonchromosomeX,onceinintron22oftheF8geneandtwiceclosertotheXqtelomere.Thisrecordrepresentsthemostcentromericcopywhichisinintron22oftheF8gene.[providedbyRefSeq].
      GeneSymbol
      • H2A.Bbd
      • H2AFB1
      PurificationMethodAffinityPurfied
      UniProtNumber
      UniProtSummaryFUNCTION:AtypicalhistoneH2AwhichcanreplaceconventionalH2Ainsomenucleosomes.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstability.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.Nucleosomescontainingthishistonearelessrigidandorganizeonly118basepairofDNAinsteadof147inclassicalnucleosomes.TheyareassociatedwithtranscriptionallyactivechromatinandexcludedformBarrbodies.Ref.4Ref.5

      SUBUNITSTRUCTURE:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Maybeincorporatedintoaproportionofnucleosomes,replacingoneormoreH2Amolecules.

      SUBCELLULARLOCATION:Nucleus.Chromosome.Note:AssociatedwiththeactiveXchromosomeandwithautosomes,whileitisabsentfromtheinactiveXchromosomeandexcludedfromBarrbodies.

      DOMAIN:ThedockingdomainisresponsiblefortheweakerheterodimerizationwithH2B.Ref.4

      MISCELLANEOUS:IncontrasttootherH2Ahistones,itdoesnotcontaintheconservedresiduesthatarethetargetofpost-translationalmodifications.

      SEQUENCESIMILARITIES:BelongstothehistoneH2Afamily.
      MolecularWeight~17kDaobserved
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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