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Millipore/17-10099 | ChIPAb+ Acetyl-Histone H4 (Lys8) - ChIP Validated Antibody and Primer Set/17-10099/25 assays25 assa
  • Millipore/17-10099 | ChIPAb+ Acetyl-Histone H4 (Lys8) - ChIP Validated Antibody and Primer Set/17-10099/25 assays25 assa

Millipore/17-10099 | ChIPAb+ Acetyl-Histone H4 (Lys8) - ChIP Validated Antibody and Primer Set/17-10099/25 assays25 assa

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货号: 17-10099
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    • Description
      CatalogueNumber17-10099
      BrandFamilyUpstate
      TradeName
      • ChIPAb+
      • Upstate
      DescriptionChIPAb+Acetyl-HistoneH4(Lys8)-ChIPValidatedAntibodyandPrimerSet
      OverviewAllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction.
      TheChIPAb+Acetyl-HistoneH4(Lys8)setincludestheAcetyl-HistoneH4(Lys8)antibody,anegativecontrolrabbitserum,andqPCRprimerswhichamplifya134bpregionofhumanHSPCA.TheAcetyl-HistoneH4(Lys8)andnegativecontrolsaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofAcetyl-HistoneH4(Lys8)-associatedchromatin.
      AlternateNames
      • H4K8Ac
      • HistoneH4(acetylK8)
      BackgroundInformationHistoneH4isoneofthe5mainhistoneproteinsinvolvedinthestructureofchromatinineukaryoticcells.FeaturingamainglobulardomainandalongNterminaltailH4isinvolvedwiththestructureofthenucleosomesofthe"beadsonastring"structure.

      TheN-terminaltailofhistoneH4protrudesfromtheglobularnucleosomecoreandcanundergoseveraldifferenttypesofepigeneticmodificationsthatinfluencecellularprocesses.Thesemodificationsincludethecovalentattachmentofmethyloracetylgroupstolysineandarginineaminoacidsandthephosphorylationofserineorthreonine.
      ProductInformation
      FormatSerum
      Control
      • IncludesnegativecontrolnormalrabbitserumantibodyandprimersspecificforhumanHSPCA.
      PresentationAnti-Acetyl-HistoneH4(Lys8)(rabbitpolyclonal).Onevialcontaining50μLofantiserumcontaining0.05%sodiumazide.Storeat-20°C.

      NormalRabbitSerum.Onevialcontaining100μLofantiserumcontaining0.05%sodiumazide.
      Storeat-20°C.

      ChIPPrimers,HSPCA.Onevialcontaining75μLof5μMofeachprimerspecificforhumanHSPCA.
      Storeat-20°C.
      FOR:GCAACAGCTACCACAGGACCA
      REV:GAGCGTGTGAAATCAACATAAAGC
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
      Applications
      ApplicationTheChIPAb+Acetyl-HistoneH4(Lys8)setincludestheAcetyl-HistoneH4(Lys8)antibody,anegativecontrolrabbitserum&qPCRprimerswhichamplifya134bpregionofhumanHSPCA.
      KeyApplications
      • WesternBlotting
      • ChromatinImmunoprecipitation(ChIP)
      ApplicationNotesChromatinImmunoprecipitation:
      SonicatedchromatinpreparedfromHeLacells(1X106cellequivalentsperIP)weresubjectedtochromatinimmunoprecipitationusingeither2µLofNormalRabbitSerumor2µLofAnti-Acetyl-HistoneH4(Lys8)andtheMagnaChIP™AKit(Cat.#17-610).
      SuccessfulimmunoprecipitationofAcetyl-HistoneH4(Lys8)associatedDNAfragmentswasverifiedbyqPCRusingChIPPrimers,humanHSPCAforapositive,andknownNegativePrimers(Martinato,F.,etal.,2008)asanegativeassay(Pleaseseefigures).
      DataispresentedaspercentinputofeachIPsamplerelativetoinputchromatinforeachampliconandChIPsampleasindicated.
      PleaserefertotheEZ-MagnaChIP™A(Cat.#17-408)orEZ-ChIP™(Cat.#17-371)protocolforexperimentaldetails.

      WesternBlotAnalysis:
      Representativelotdata.
      SodiumButyrate-treatedHeLaacidextractwasresolvedbyelectrophoresis,transferredtoPVDFmembraneandprobedwithAnti-AcetylHistoneH4(Lys8)(1:1000dilution).Todemonstratespecificity,serumwaspreincubatedwithmodifiedHistoneH4peptides:
      Lane1:Nopeptide
      Lane2:acetyl-HistoneH4(K5)(Cat.#:12-343)
      Lane3:acetyl-HistoneH4(K8)(Cat.#:12-344)
      Lane4:acetyl-HistoneH4(K12)(Cat.#:12-345)
      Lane5:acetyl-HistoneH4(K16)(Cat.#:12-346)
      Lane6:acetyl-HistoneH4(K5,8,12,16)(Cat.#:12-353)
      Lane7:HistoneH4(Cat.#:12-347)
      Proteinswerevisualizedusingadonkeyanti-rabbitIgGconjugatedtoHRPandvisualizedusingachemiluminescencedetectionsystem(Pleaseseefigures).

      Immunoprecipitation:5μLofapreviouslotimmunoprecipitatedacetylHistoneH4from1mgofwildtypewholecellyeastlysatesbutnotfromayeaststraincontainingaHistoneH4Lys8Rsubstitution.
      BiologicalInformation
      ImmunogenSyntheticpeptidecorrespondingtoyeastHistoneH4acetylatedatlysine8.
      HostRabbit
      SpecificityRecognizesacetyl-HistoneH4(Lys8).
      SpeciesReactivity
      • Human
      • Yeast(S.cerevisiae)
      SpeciesReactivityNoteDemonstratedtoreactwithYeastandhuman.Broadspeciescross-reactivityisexpectedbasedon100%sequencehomology.
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryHistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Twomoleculesofeachofthefourcorehistones(H2A,H2B,H3,andH4)formanoctamer,aroundwhichapproximately146bpofDNAiswrappedinrepeatingunits,callednucleosomes.Thelinkerhistone,H1,interactswithlinkerDNAbetweennucleosomesandfunctionsinthecompactionofchromatinintohigherorderstructures.ThisgeneisintronlessandencodesamemberofthehistoneH4family.TranscriptsfromthisgenelackpolyAtailsbutinsteadcontainapalindromicterminationelement.Thisgeneisfoundinthelargehistonegeneclusteronchromosome6.
      GeneSymbol
      • HIST1H4A
      • H4/J
      • HIST1H4F
      • HIST1H4L
      • H4FN
      • H4FA
      • H4FH
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • HIST2H4
      • H4FB
      • H4/K
      • HIST1H4I
      • H4/N
      • HIST1H4B
      • H4FE
      • H4/M
      • H4/a
      • HIST1H4E
      • HIST4H4
      • HIST2H4A
      • H4FC
      • H4FI
      • H4/H
      • HIST1H4C
      • H4/G
      • HIST1H4K
      • H4FJ
      • H4FD
      • H4/I
      • H4/B
      • H4/D
      • H4/C
      • HIST1H4J
      • HIST1H4D
      • H4/E
      Modifications
      • Acetylation
      PurificationMethodUnpurified
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P62805#Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstABIlity.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.
      SIZE:103aminoacids;11367Da
      SUBUNIT:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA.
      SUBCELLULARLOCATION:Nucleus.
      PTM:SymmetricdimethylationonArg-4bythePRDM1/PRMT5complexmayplayacrucialroleinthegerm-celllineage(Bysimilarity).&UbiquitinatedbytheCUL4-DDB-RBX1complexinresponsetoultravioletirrADIation.ThismayweakentheinteractionbetweenhistonesandDNAandfacilitateDNAaccessibilitytorepairproteins.
      SIMILARITY:SwissProt:P62805##BelongstothehistoneH4family.
      MolecularWeight~11kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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