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Millipore/17-10047 | ChIPAb+ Histone H4 - ChIP Validated Antibody and Primer Set, rabbit monoclonal/17-10047/25 assays25
  • Millipore/17-10047 | ChIPAb+ Histone H4 - ChIP Validated Antibody and Primer Set, rabbit monoclonal/17-10047/25 assays25

Millipore/17-10047 | ChIPAb+ Histone H4 - ChIP Validated Antibody and Primer Set, rabbit monoclonal/17-10047/25 assays25

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    • Description
      CatalogueNumber17-10047
      BrandFamilyUpstate
      TradeName
      • ChIPAb+
      • Upstate
      DescriptionChIPAb+HistoneH4-ChIPValidatedAntibodyandPrimerSet,rabbitmonoclonal
      OverviewAllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction.
      TheChIPAb+HistoneH4setincludestheHistoneH4antibody,anegativecontrolantibody(normalrabbitIgG),andqPCRprimerswhichamplifya110bpregionofhumanβ-Globin.TheHistoneH4andnegativecontrolantibodiesaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofHistoneH4-associatedchromatin.
      AlternateNames
      • H4
      • HistoneH4
      • H4histonefamily,memberA
      • histone1,H4a
      • histonecluster1,H4a
      BackgroundInformationHistoneH4isoneofthe5mainhistoneproteinsinvolvedinthestructureofchromatinineukaryoticcells.FeaturingamainglobulardomainandalongNterminaltailH4isinvolvedwiththestructureofthenucleosomesofthe"beadsonastring"structure.
      ProductInformation
      FormatPurified
      Control
      • Includesnegativecontrolantibody(normalrabbitIgG)andprimersspecificforhumanβ-Globin.
      PresentationAnti-HistoneH4(rabbitmonoclonalIgG).Onevialcontaining50µLofproteinApurifiedrabbitmonoclonalIgGsupernatantinbuffercontaining0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%sodiumazidebeforeadditionofglycerolto40%.Storeat-20°C.

      NormalRabbitIgG.Onevialcontaining75µgofnormalrabbitIgGin75µLstoragebuffer.Storeat-20°C.

      ChIPPrimers,β-Globin.Onevialcontaining75μLof5μMofeachprimerspecificforhumanβ-Globin.Storeat-20°C.
      FOR:AGGACAGGTACGGCTGTCATC
      REV:TTTATGCCCAGCCCTGGCTC

      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.
      Applications
      ApplicationThisChIPAb+HistoneH4-ChIPValidatedAntibody&PrimerSetconvenientlyincludestheantibody&thespecificcontrolPCRprimers.
      KeyApplications
      • WesternBlotting
      • ChromatinImmunoprecipitation(ChIP)
      ApplicationNotesChromatinImmunoprecipitation:
      SonicatedchromatinpreparedfromHeLacells(1X106cellequivalentsperIP)weresubjectedtochromatinimmunoprecipitationusing2µLofeithernormalrabbitIgGor2µLofAnti-HistoneH4antibodyandtheMagnaChIP™AKit(Cat.#17-610).
      SuccessfulimmunoprecipitationoftotalHistoneH4associatedDNAfragmentswasverifiedbyqPCRusingChIPPrimers,β-Globin,aswellasGAPDHpromoterControlPrimers,(Pleaseseefigures).
      DataispresentedaspercentinputofeachIPsamplerelativetoinputchromatinforeachampliconandChIPsampleasindicated.

      PleaserefertotheEZ-MagnaChIP™A(Cat.#17-408)orEZ-ChIP™(Cat.#17-371)protocolforexperimentaldetails.

      WesternBlotAnalysis:
      RepresentativeLotData.
      HeLaAcidExtract(Lane1and3),HeLaAcidExtractfromsodiumbutyratetreatedcells(Lane2),HeLaAcidExtractfromcolcemidtreatedcells(Lane4)andrecombinanthistoneH4(Lane5)wereresolvedbyelectrophoresis,transferredtoPVDFmembranesandprobedwithAnti-HistoneH4(1:1,000dilution).
      ProteinswerevisualizedusingaDonkeyanti-rabbitIgGconjugatedtoHRPandvisualizedusingachemiluminescencedetectionsystem.(Pleaseseefigures).
      BiologicalInformation
      ImmunogenKLH-conjugatedsyntheticpeptidecorres-pondingtoaminoacids17-28ofHistoneH4.
      Epitopea.a25-28
      HostRabbit
      SpecificityRecognizesHistoneH4.
      SpeciesReactivity
      • Human
      SpeciesReactivityNoteHuman.Widerangeofcross-reactivityexpectedbasedonsequencehomology.
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryHistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Thisstructureconsistsofapproximately146bpofDNAwrappedaroundanucleosome,anoctamercomposedofpairsofeachofthefourcorehistones(H2A,H2B,H3,andH4).Thechromatinfiberisfurthercompactedthroughtheinteractionofalinkerhistone,H1,withtheDNAbetweenthenucleosomestoformhigherorderchromatinstructures.ThisgeneisintronlessandencodesamemberofthehistoneH4family.TranscriptsfromthisgenelackpolyAtails;instead,theycontainapalindromicterminationelement.Thisgeneisfoundinahistoneclusteronchromosome1.Thisgeneisoneoffourhistonegenesintheclusterthatareduplicated;thisrecordrepresentsthetelomericcopy.[providedbyRefSeq].
      GeneSymbol
      • HIST1H4A
      • H4/J
      • HIST1H4F
      • HIST1H4L
      • H4FN
      • H4FA
      • H4FH
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • HIST2H4
      • H4FB
      • H4/K
      • HIST1H4I
      • H4/N
      • HIST1H4B
      • H4FE
      • H4/M
      • H4/a
      • HIST1H4E
      • HIST4H4
      • HIST2H4A
      • H4FC
      • H4FI
      • H4/H
      • HIST1H4C
      • H4/G
      • HIST1H4K
      • H4FJ
      • H4FD
      • H4/I
      • H4/B
      • H4/D
      • H4/C
      • HIST1H4J
      • HIST1H4D
      • H4/E
      PurificationMethodProteinAPurfied
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P62805#Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstability.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.
      SIZE:103aminoacids;11367Da
      SUBUNIT:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA.
      SUBCELLULARLOCATION:Nucleus.
      PTM:SymmetricdimethylationonArg-4bythePRDM1/PRMT5complexmayplayacrucialroleinthegerm-celllineage(Bysimilarity).&UbiquitinatedbytheCUL4-DDB-RBX1complexinresponsetoultravioletirrADIation.ThismayweakentheinteractionbetweenhistonesandDNAandfacilitateDNAaccessibilitytorepairproteins.
      SIMILARITY:SwissProt:P62805##BelongstothehistoneH4family.
      MolecularWeight~11kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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