Description
Lipofectamine®LTXReagentisaplasmidtransfectionreagentthatofferstherightbalanceofpotencyandgentlenessforyourcells,resultinginthehighestposs
IBLetransfectionefficienciesandvi
ABIlities.Lipofectamine®LTXReagentisanew,advancedsolutionforgeneexpressioninbothcommonandchallengingcelltypes.NootherplasmidDNA-specifictransfectionreagentcanmatchtheefficiency,convenience,andgentlenessofLipofectamine®LTXReagentfortransfectionofprimary,challenging,andsensitivecelllines.Lipofectamine®LTXReagentissynthesizedfrom100%animalorigin-freecomponents,makingiteasytovalidatetheabsenceofzoonoticdiseases,suchasBSEorviruses,inresearchexperimentsorcelllines.AcertificateoforiginmaybeobtainedbycontactingTechnicalSupport.
Lipofectamine®LTXReagentoffers:•Superiordelivery–transfectabroadrangeofcelltypes,includingprimarychallengingandsensitivecells,withhighefficiency
•Superiorperformance–combinehightransfectionefficiencywithmaximal(>90%)cellviability
•Optimizedprotocols–rapid,simpleprotocolsprovidedformostcelllines
•Ahighlyeffectivereagenttogeneratestablecelllines
TransfectPrimaryCellsEfficientlyPrimarycellsareoftendifficulttotransfect.Lipofectamine®LTXReagent,whencombinedwithPLUS™Reagent,providesthehighestleveloftransfectioninprimarycellsofanylipid-orpolymer-basedreagent.ManyprimarycelltypeshavebeensuccessfullytransfectedwithLipofectamine®LTXincludingprimaryneural
Progenitorcells.
SuperiorDeliveryintoChallengingCellsWhenitcomestoachievinghighlevelsofproteinexpressioninRAW267.3macrophages,Lipofectamine®LTXReagentoutperformscompetingtransfectionreagents(seeFigure4).Achievinghigh-leveltransgeneexpressionincellsthataretr
ADItionallyrefractorytotransfectionallowsyoutoobtainresultsthatwerepreviouslyunattainable.
BroadCompatibilitywithCellLinesInadditiontobeingexcellentfortransfectingprimaryandchallengingcells,Lipofectamine®LTXReagentprovidesyouwiththemostversatileapproachtotransfectingestablishedcellslines,includingthosethataresensitivetoothertransfectionreagents.UseourTransfectionSelectionTooltohelpyouselectthebesttransfectionconditionsforthecellswithwhichyouwork.
StableTransfectionLipofectamine®LTXstrikestherightbalancebetweenpotencyandgentleness,givingyouthehighestpossiblepercentageofsuccessfullytransfectedcells.ThismakesLipofectamine®LTXthequickestandeasiestreagentforestablishingstabletransfectantsfromchallengingorrefractorycelltypes.
Simple,Rapid,Serum-compatibleProtocolLipofectamine®LTXReagentoffersastreamlinedprotocol–noneedtoremovetransfectioncomplexesorchange/addmediumfollowingtransfection.Asimple,optimizationprotocolhelpsyougetthebestresultsquicklysoyou"llspendlesshands-ontime,andgetfasterresults.
Lipofectamine®LTXandPLUS™Reagentisavailableinthefollowingsizes:•0.1mlwith40µlPLUS™Reagent
•0.3mlwith0.25mlPLUS™Reagent
•1.0mlwith0.85mlPLUS™Reagent
•15ml(purchasePLUS™Reagentseparately)
ForResearchUseOnly.Notforuseindiagnosticprocedures.