Pierce/Dynabeads M-270 Epoxy/60 mg/14301

Description

Dynabeads®M-270Epoxybeadsare2.8µmsuperparamagneticbeadscontainingsurfaceepoxygroups.Thebeadscovalentlybindprimaryaminoandsulfhydrylgroupsinproteinsandpeptides,makingthemidealforcouplingantibodies,peptides,intactproteins,andfunctionalenzymes.Thesehydrophylic,neutralpHbeadsexhibitextremelylownonspecificbindingofproteinsanddyes,whichreducestheneedforblockingagents.Also,Dynabeads®M-270EpoxyBeadsdonotcontainTween®detergent,andsoareidealforuseinmassspectrometry(MS)analysis.

•Idealforimmunoprecipitation(IP)ofproteinsandproteincomplexes
•Gentle,yetrapidmagneticseparationandshortincubationtimesallowidentificationoftransientandlABIlecomplexes
•Covalentantibody-couplingtotheDynabeads®avoidsco-elutionoftheantibodywiththetargetprotein

Adaptableforvariousapplications
FastsurfacebindingkineticsandrapidmagneticseparationcharacteristicsmakeDynabeads®M-270Epoxybeadstheidealchoiceforapplicationssuchasimmunoprecipitation/purificationofproteinsandproteincomplexes,couplingfunctionalenzymestothebeadsurfacefordownstreamassays,andidentifyingproteinbindingpartners.Capturedproteinsandproteincomplexesareeasilyseparated,washed,andelutedusingaDynaMag™magnet,andthemagneticseparationpropertiesoftheDynabeads®.

Couplingprocedure
Covalentcouplingisperformedovernightbyincubatingthedesiredligand(commonlyantibodies,peptides,orproteins)withDynabeads®M-270Epoxybeads.Thebindingcapacityvariesdependingontheligandused,butistypically5–10µgIgGpermilligramofbeads.CouplingoccursatneutralpHandhighsaltconcentration.Thecouplingreactioncanbeperformedoverabroadrangeoftemperaturestomatchthestabilitycharacteristicsofyourligand.

Uponcompletionoftheligandcouplingstep,thereactivemoietiesontheDynabeads®M-270Epoxybeadsaredeactivatedtopreventfurthercoupling.Oncethecouplingreactioniscompleted,thesenonporous,hydrophilic,pHneutralbeadsexhibitextremelylownonspecificbinding.

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