GoldBio/Noggin, Murine/20 μg/1380-09-20

GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!

Recombinant Murine NOGNoggin

Noggin belongs to a group of diffusible proteins that bind to ligands of the TGF-β (Transforming Growth Factor) family and regulate their activity by inhibiting their access to signaling receptors. Noggin was originally identified as a BMP4 antagonist whose action is critical for proper formation of the head and other dorsal structures. Noggin has been shown to modulate the activities of other BMPs including BMP2, 7, 13 and 14. Targeted deletion of noggin in mice results in prenatal death and recessive phenotype displaying a severely malformed skeletal system. Transgenic mice overexpressing noggin in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis.

Formulation: Sterile filtered whitelyophilized powder. Purified and tested for use in cell culture

Sequence: MQHYLHIRPAPSDNLPLVDL IEHPDPIFDP KEKDLNETLL RSLLGGHYDP GFMATSPPED RPGGGGGPAG GAEDLAELDQLLRQRPSGAM PSEIKGLEFS EGLAQGKKQR LSKKLRRKLQ MWLWSQTFCP VLYAWNDLGS RFWPRYVKVGSCFSKRSCSV PEGMVCKPSK SVHLTVLRWR CQRRGGQRCG WIPIQYPIIS ECKCSC

MW: ~46.4 kDa (2x206 amino acid)(A non-disulfide linked homodimer consistingof two 206 amino acid polypeptide chains)

Purity: >95% by SDS-PAGEand HPLC analyses

Biological Activity: Fully biologically active compared to standard. The ED₅₀ as determined by inhibiting BMP-4 induced alkaline phosphatase production of murine ATDC5 cells is less than 2 ng/ml, corresponding to a specific activity of >5.0×10⁵ IU/mg in the presence of 5 ng/ml BMP-4.

Accession Number:P97466

MGI ID: 104327

Source: Escherichia coli

Storage/Handling: This lyophilizedpreparation is stable at 2-8°C, but should be kept at -20°C for long termstorage. The reconstituted sample can be apportioned into working aliquots andstored at -80 °C for up to 6 months. Avoid repeated freeze/thaw cycles.

Reconstitution: The sample shouldbe briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in aqueous buffer containing 10mM HAc to a concentration of<0.25 mg/ml. Reconstituted solutions are stable for up to one week at2-8°C.Stock solutions should bealiquoted and stored at -80°C. Further dilutions should be made in appropriatebuffered solutions

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OMIM

NOT FOR HUMAN USE!