Non-activatedcarboxylicacid,ananalogofCy5®freecarboxylicacid.ContainsCyanine5fluorophore.
Thisdyehaslimitedwatersolubility,butcanbedissolvedinmixturesofwaterwithorganicphase(DMF,DMSO,alcohols)toobtainusefulconcentrationsofthematerialinsolution.Water-solubleversionisavailable.
Thismoleculecanbeconsiderednon-reactivedyefortheuseincontrolsamples,andforinstrumentcalibration.Forcouplingwithaminesandproteinlabeling,considerusingCyanine5NHSester,orwater-solublesulfo-Cyanine5NHSester.
Generalproperties
Appearance: | darkbluepowder |
Molecularweight: | 519.12 |
Molecularformula: | C32H39ClN2O2 |
Solubility: | solubleinorganicsolvents(DMF,DMSO,dichloromethane),insolubleinwater |
Qualitycontrol: | NMR1H,HPLC-MS(95%) |
Storageconditions: | Storage:24monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight.Desiccate. |
Spectralproperties
Excitationmaximum,nm: | 646 |
ε,L⋅mol−1⋅cm−1: | 250000 |
Emissionmaximum,nm: | 662 |
Fluorescencequantumyield: | 0.2 |
CF260: | 0.03 |
CF280: | 0.04 |