TheCD34
+CD38
–CellIsolationKit,humanhasbeendevelopedtoisolateaCD38
–subsetofCD34
+cells,i.e.,primitivehematopoieticstemand
Progenitorcells,fromcordblood,bonemarrow,orapheresis.
TheCD34
+CD38
–CellIsolationKithasbeendevelopedfortheisolationofthemoreprimitiveCD38
–subpopulationofCD34
+cells.CD34isawell-established
Markerofhumanhematopoieticstemandprogenitorcellsandadditionallyexpressedonhemangioblasts,endothelialprogenitorcells,andmatureendothelialcells.CD38isabsentonmostprimitiveCD34
+stemcellsandupregulatedonCD34
+cellsuponlymphocytecommitment.CD34
+CD38
-cellsareisolatedinatwo-stepisolationprotocol.First,theCD34
+cellsarepositivelyenrichedusingCD34MultiSortMicroBeads.Subsequently,theMultiSortMicroBeadsareenzymaticallyreleasedfromtheCD34antibody.TheisolatedCD34
+arethenlabeledwithCD38MicroBeadstodepleteCD38
+cellsinasecondseparationstep.
LSColumns.