Capacity:for2×109totalcellsOverview
InmiceF4/80isawell-established,yetnotexclusive,macrophage
Marker.Anti-F4/80MicroBeadsUltraPurehavebeenoptimizedfortherapidandsimpleisolationofmousemacrophagesfromsingle-cells
USPensionsfromlymphoidandnon-lymphoidtissues.TheUltraPureMicroBeadsgreatlyimproverecoveryandpurityofthesortedpopulationbyspecificallyenrichingviablecells.
Details
F4/80isamemberofepidermalgrowthfactor(EGF)-transmembrane7(TM7)familyandconsistsofaseven-spantransmembranemoleculewithalargeextracellular,multipleEGFmodule–containing,domain.F4/80isconsideredasoneofthemostspecificcell-surfacemarkersformurinemacrophages.ConstitutiveandhighexpressionofF4/80isfoundonmostresidenttissuemacrophages,includingspleen,microgliainthebrain,Kupffer’scellsintheliver,andLangerhanscellsintheskin.Inaddition,theexpressionofF4/80canalsoberegulateddependingonthephysiologicalstatusofthecell.
Downstreamapplications
MacrophagesisolatedwithAnti-F4/80MicroBeadsUltraPurecanbeusedforphenotypicalandfunctionalanalyses,e.g.cytokinesecretion,phagocytosis,polarization,andtoleranceinductionindifferentexperimentalmurinemodels.
Columns
MS,LS,orauto
MACS®Columns.