Capacity:forupto2.5×108totalcellsOverview
ThePSC-DerivedCardiomyocyteIsolationKitallowsthemagneticseparationofvitalcardiomyocytesdifferentiatedfromhumanpluripotentstemcells.Theprocedureallowstheenrichmentofcardiomyocytesinlessthananhour.
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Backgroundinformation
Pureandwell-characterizedcardiomyocytesderivedfromhumanpluripotentstemcells(PSCs)areofhighinterestforcardiovasculardiseasemodeling,drugsafetystudies,anddevelopmentofcellreplacementstrategies.AlthoughseveralprotocolsforcardiacdifferentiationofPSCshavebeendeveloped,therearestillmajorlimitations,includingclone-to-cloneandexperiment-to-experimentvariationindifferentiationefficacy.TocircumventtheselimitationsthePSC-DerivedCardiomycoyteIsolationKit,humanhasbeendeveloped.ItenablesthepurificationofPSC-derivedcardiomyocytestohighpuritiesindependentofthedifferentiationefficiency,thestemcellclone,thedifferentiationtimepointanddifferentiationprotocol.TheisolationofPSC-derivedcardiomyocyteshassuccessfullybeentestedforvariousdifferentiationefficienciesandtimepointsofdifferentiation.