Content:5×100µgin50µLOverview
OligodeoxyribonucleotidescontainingCpGmotifs(CpGODNs)arewidelyusedforactivationofimmunecells,suchashumanPBMCs,murinesplenocytesorisolatedimmunecells,e.g.,BcellsandpDCs.TheyalsoactivatesignallinginTLR9-expressingrecombinantcelllines.
ODN1826stronglyactivatesmurineTLR9.ItisaB-classODNforactivationofBcells,inductionofIl-6secretion,andactivationofNF-kB-signallingpathways.
Details
Backgroundinformation
TLR9isaprominentmemberofthetoll-like-receptor(TLR)familyrecognizingpathogen-associatedmolecularpatterns.TLR9recognizesspecificallyunmethylatedCpGmotifsinbacterialDNAle
ADIngtoactivationofimmunecells
1,
2.Theseeffectscanbemimickedbyshortsyntheticaloligodeoxyribonucleotides(ODNs)containingunmethylatedCpGmotifs
3.
SeveralclassesofCpGODNhavebeenidentifiedandcanbedistinguishedbytheireffectsoncertaincelltypes.A-classODNscontaining5’and3’G-richstretchesinducehighlevelsoftypeIIFNbutshowlowactivationofBcellproliferation.B-classODNsactivateBcellsandTLR9-dependentNFkBsignallinginrecombinantcelllinesbutshowlowinductionofIFN-α.C-classODNinducehighamountsofIFN-αandactivateBcells.TherecentlydiscoverednewP-ClassODNsshowsimilarbutsuperiorpropertiestoC-classODNs.
ODN1826isaB-classODNthatstronglyactivatesmurineTLR9.
Applications
CpGODNscanbeusedforactivationofimmunecells,suchashumanperipheralbloodmononuclearcells(PBMCs),murinesplenocytes,orisolatedimmunecells(e.g.,BcellsandpDCs).CpGODNscanbeusedtoactivatesignalinginTLR9-expressingrecombinantcelllines.