CloneREA402recognizesthesarcomericα-actininantigen,a100kDaactin-bindingprotein,whichoccupiesastrategicroleintheassemblyandmaintenanceofstressfibersofnon-musclecellsandthemyofibrilsofmusclecells.α-actininshavebeenhighlyconservedthroughoutevolutionandarelargelycollinearproteinsthatsharethreeconservedfunctionaldomains.Functionally,α-actininsformantiparallelhomodimerswiththeactin-bindingdomainsoneachendofthemolecule,allowingcrosslinkingofactinmolecules.Twoisoformsofα-actininhavebeenidentified,amuscle-specificsarcomericisoformandanon-sarcomericisoform.Themajor–andbestcharacterized–functionaldifferenceamongα-actininisoformsiscalciumsensitivityforactin-binding.Thebindingofα-actinintoactinbynon-musclecytoskeletalisoformsisdependentonthecalciumconcentration,whereasthisinteractionisindependentofcalciumconcentrationinsarcomericstriatedandsmoothmuscleisoforms.Studiesonskeletalandcardiaccellshavelocalizedsarcomericα-actinintoZ-bandsinstriatedmyofibrils,toprecursorI-Z-I-likecomplexesinmuscleundergoingmyofibrillogenesis,andtovinculin-positiveadhesionplaquesandadherensjunctions.
Additionalinformation:CloneREA402displaysneglig
IBLebindingtoFcreceptors.