Capacity:forupto5×108totalcellsOverview
TheNeonatalCardiacEndothelialCellIsolationKit,rathasbeendevelopedforthetwo-stepisolationofvitalcardiacendothelialcellsfromneonatalrathearts(P0-P3).Enrichedcardiacendothelialcellsarefullyfunctionalandcanbeusedforvariousdownstreamapplications.
Details
Backgroundinformation
Besidescardiomyocytesandfibroblasts,endothelialcellsrepresentthethirdmostfrequentcellpopulationintheneonatalmouseheart.Endothelialcellsformtheinnerlayerofbloodvesselsandplayakeyroleinvasculardevelopment,homeostasisandremodeling.TheNeonatalCardiacEndothelialCellIsolationKit,rathasbeendesignedforenrichmentofcardiacendothelialcellsfromdissociatedneonatalrathearts.Theisolationofcardiacendothelialcellshassuccessfullybeentestedusingneonatalratheartsfrompostnatalday0today3(P0-P3).Foroptimaluse,theNeonatalCardiacEndothelialCellIsolationKitshouldbeusedincombinationwiththeNeonatalHeartDissociationKit,mouseandrat(#130-098-373).
Columns
Forthefirstmagneticseparation(depletion):LDColumns.Forthesecondmagneticseparation(positiveselection):MSColumns.