Granulocytemacrophagecolony-stimulatingfactor(GM-CSF)isahematopoieticgrowthfactorthatisessentialforproliferationanddevelopmentofgranulocyteandmonocyte/macrophage
Progenitors.Italsofunctionsasagrowthfactorforerythroidandmegakaryocyticprecursorcellsinconjunctionwitherythropoietin.GM-CSFissecretedbyvariouscelltypesincludingTcells,macrophages,endothelialcells,andfibroblastsinresponsetoinflammatorystimuliandcytokines.Inaddition,GM-CSFstronglychemoattractsneutrophilsandeosinophilsandenhancestheeffectorfunctionsofneutrophilsandmacrophages.
TheGM-CSFSecretionAssaycanbeusedforthedetectionofviableGM-CSF-secretingleukocytes.Antigen-specificTcellimmunity,e.g.ininfection,autoimmunityandallergy,canbemonitoredandanalyzedwiththisdetectionkit,aswellasenumerationandphenotypicorfunctionalcharacterizationofGM-CSF-secretingcells.TheGM-CSFSecretionAssay–DetectionKit(PE)canbecombinedwithasecondDetectionKit(FITCorAPC)toanalyzeco-expressedcytokines.