CloneREA338recognizesthehumanoctamer-bindingtranscriptionfactor3/4isoformA(Oct3/4A)andthemouseOct3/4.Oct3/4isahomeodomaintranscriptionfactorbelongingtothePOUtranscriptionfactorfamilyandisalsoknownasPOUdomainclass5transcriptionfactor1(POU5F1).Oct3/4iscriticalforthemaintenanceoftotipotencyinprimateblastomeresandforpluripotencyoftheinnercellmassofdevelopingmammalianembryos.ThiscriticalroleofOct3/4ismaintainedinembryonicstemcells,thedevelopinggermline,andgermlinetumors,suchasseminomasandembryonicteratocarcinomas.Wheninducingpluripotencyindifferentiatedcells,Oct3/4appearstobeanessential,butnottheonlyrequiredtranscriptionfactor.Oct3/4actsasatranscriptionalactivatorofgenesinvolvedinmaintenanceofundifferentiatedstateandasarepressorofdifferentiation-specificgenes.Inhumanitencodestwochiefisoformsthataregeneratedbyalternativesplicing–Oct3/4AandOct3/4b.OnlytheOct3/4AproteinconsequentlycontainstheuniqueN-terminaltransactivationdomainthatregulatesDNAbindingofOct3/4Atotargetpromoters.ItisonlythisfulllengthOct3/4Aprotein–thatlocalizestothenucleus,bindstoOct3/4-regulatedpromoters,andcontributestothemaintenanceofpluripotencyandself-renewal.
Additionalinformation:CloneREA338displaysneglig
IBLebindingtoFcreceptors.