Capacity:forupto2.5×108totalcellsOverview
TheNeonatalCardiacEndothelialCellIsolationKit,mousehasbeendevelopedforthetwo-stepisolationofvitalmurinecardiacendothelialcells(P0-P3).Enrichedcardiacendothelialcellsarefullyfunctionalandcanbeusedforvariousdownstreamapplications.
Details
Backgroundinformation
Besidescardiomyocytesandfibroblasts,endothelialcellsrepresentthethirdmostfrequentcellpopulationintheneonatalmouseheart.Endothelialcellsformtheinnerlayerofbloodvesselsandplayakeyroleinvasculardevelopment,homeostasisandremodeling.TheNeonatalCardiacEndothelialCellIsolationKit,mousehasbeendesignedforenrichmentofcardiacendothelialcellsfromdissociatedneonatalmousehearts.Theisolationofcardiacendothelialcellshassuccessfullybeentestedusingneonatalmouseheartsfrompostnatalday0today3(P0-P3).Foroptimaluse,theNeonatalCardiacEndothelialCellIsolationKitshouldbeusedincombinationwiththeNeonatalHeartDissociationKit,mouseandrat(#130-098-373).
Columns
Forthefirstmagneticseparation(depletion):LDorauto
MACS®Columns.Forthesecondmagneticseparation(positiveselection):MSorautoMACSColumns.