Capacity:for100testsOverview
MACSPlexAssaysaredesignedfordeterminingconcentrationsofsolubleanalytesinasinglesample.TheanalysisisbasedonMACSPlex(MPx)CaptureBeads,whichdisplaydefinedfluorescencepropertiesandcanbeidentifiedusingstandardflowcytometrytechniques.
MPxCaptureBeadswithintheMACSPlexCytokine12Kit,humanandMACSPlexCytokine10Kit,mousecontainacocktailofvariousfluorescentlylabeledbeadpopulations,eachcoatedwithaspecificantibodyreactingwithoneoftherespectivecytokineswithinthesample.
IncombinationwiththeExpressModesoftheMACSQuant®Analyzers(currentlyinstallExpressModes_26.1.12747)theMACSPlexCytokineKitsareoptimizedforautomatedmeasurementofbeads.Theysimplifyflowcytometricanalysisviapredefinedexperimentsettingsaswellasacquisitionandanalysistemplates.Theyapplyanautomatedgatingstrategytopopulationsofinterestthatwillbeautomaticallyadjustedforeachdatafileindividuallytoachieveoptimalresults.
Details
MACSPlexCytokine12CaptureBeads,humanandMACSPlexCytokine10CaptureBeads,mouseconsistofbeadpopulationsthathavebeencoatedwithcaptureantibodiesspecificforcytokines.ThebeadpopulationscanbedistinguishedbydifferentfluorescenceintensitiesdetectedintheB1andB2channeloftheMACSQuant®AnalyzerandMACSQuantAnalyzer10(aswellasrespectivechannelsofcomparableflowcytometers).MACSPlexCytokineCaptureBeadsareaddedtounknownsamplesandtoserialdilutionsoftheMACSPlexCytokineStandard.Duringa2-hourincubationperiod,thecytokinesarecapturedbytheMACSPlexCaptureBeads.Subsequently,MACSPlexCytokineDetectionReagentcontainingamixtureofAPC-conjugatedanti-cytokineantibodies,isaddedinordertoformsandwichcomplexesduringa1-hourincubationperiod.Standardcurvesforeachofthecytokinesaregenerated.ThemedianoftheAPCfluorescenceofeachcapturebeadpopulationgivestheconcentrationofeachcytokineintheunknownsamples.
Applications
MACSPlexCytokine12Kit,humanhasbeendevelopedforthesimultaneousflowcytometricdetectionofthesolublehumancytokinesGM-CSF,IFN-α,IFN-γ,IL-2,IL-4,IL-5,IL-6,IL-9,IL-10,IL-12p70,IL-17A,andTNF-αinasinglesample.Thekithasbeenoptimizedforusewithserum,plasma,andcellculturesupernatants.
MACSPlexCytokine10Kit,mousehasbeendevelopedforthesimultaneousflowcytometricdetectionofthesolublemousecytokinesGM-CSF,IFN-γ,IL-2,IL-4,IL-5,IL-10,IL-12p70,IL-17A,IL-23,andTNF-αinasinglesample.Thekithasbeenoptimizedforusewithserum,plasma,andcellculturesupernatants.