CloneDA03-22H2.8reactswiththehumanleucine-richrepeat-containingG-protein–coupledreceptor(LGR)LGR5butnotwithitsclosehomologuesLGR4orLGR6.LGR5associateswithWnt-receptorsandactasR-spondinreceptortherebyplayingacentralroleinthemodulationofWnt/β-cateninsignalinginnormalandneoplasticstemcells.Initiallydescribedasahighlyspecific
Markerforstemcellsinthesmallintestine,colon,hairfollicle,stomach,andduringkidneydevelopment,LGR5positivecellswerealsoshowntobecrucialduringthedevelopmentandprogressionofcancer.ItwasshownthatLGR5positivecryptstemcellsarethecells-of-origininintestinalcancerandthatCSCsinhumancolorectalcancercanbeidentifiedandisolatedbasedonLGR5expression.