Capacity:forupto2×108totalcellsOverview
ThedirectAnti-TRA-1-60MicroBeadsandtheindirectAnti-TRA-1-60MicroBeadKithavebeendevelopedforthepositiveselectionofhumanpluripotentstemcellsbasedontheexpressionoftheTRA-1-60antigen.
ThedirectAnti-TRA-1-60MicroBeadsoffera10minisolationprocedure,minimizingcellhandlingtime.
Details
Backgroundinformation
TheAnti-TRA-1-60monoclonalantibodyreactswithapluripotentstemcell–specificantigenexpressedonundifferentiatedhumanembryonicstem(ES)cells,inducedpluripotent(iPS)cells,embryonalcarcinoma(EC)cells,andembryonicgerm(EG)cells.2,3TheexpressionofTRA-1-60onhumanEScellsisdown-regulatedupondifferentiation.TheAnti-TRA-1-60antibodyrecognizesaneuraminidase-resistantcarbohydrateepitopeexpressedonpodocalyxin,amemberoftheCD34-relatedfamilyofsialomucins.Podocalyxinisatransmembraneglycoprotein,whichhasbeenimplicatedinthedevelopmentofaggressivenessinavarietyofcancersincludingbreastcancerandprostatecancer.
Applications
- PositiveselectionofundifferentiatedTRA-1-60+pluripotentstemcells,forexample,humanESandiPScells.
- EfficientenrichmentofiPScellsduringorafterreprogramming1,5.
Columns
MSorauto
MACS®ColumnsforusewithAnti-TRA-1-60MicroBeads.
LSorautoMACSColumnsforusewiththeAnti-TRA-1-60MicroBeadKit.