Capacity:for2×109totalcellsOverview
TheCD34MicroBeadKitUltraPureisthelatestdevelopmentforpositiveselectionofCD34+hematopoieticstemand
Progenitorcells.ItissuitedforallroutineCD34cellisolationsandistheidealchoicefordebris-rich,lowfrequencysamples.
Details
Backgroundinformation
CD34isawell-established
Markerofhumanhematopoieticstemandprogenitorcellsandadditionallyexpressedonhemangioblasts,endothelialprogenitorcells,andmatureendothelialcells.
TheCD34MicroBeadKitUltraPurecontainsMicroBeadsdirectlyconjugatedtoCD34antibodiesformagneticlabelingofCD34-expressingcellsfromperipheralblood,cordblood,bonemarrow,orapheresisharvest.Hematopoieticprogenitorspresentatafrequencyof0.05-0.2%amongperipheralbloodmononuclearcells(PBMCs),0.1-0.5%amongcordbloodmononuclearcells(MNCs)or0.5-3%amongbonemarrowMNCscanberapidlyandefficientlyenriched.
TheCD34MicroBeadKitUltraPureissuitedforallroutineCD34+cellisolations.Inaddition,itsuniqueformulationprovidesparticularadvantageswithdebris-richsamplematerial.
ControlstainingofcellsisolatedwiththeCD34MicroBeadKitrequiresamonoclonalCD34antibodyotherthanQBEND/10,e.g.,cloneAC136.
Downstreamapplications
- CD34+cellexpansioninculture1
- Lentiviraltransduction1
- Molecularanalysisofleukemicstemcells,e.g.AMLblastsorCD34+myelofibrosissamples2
- InvitrodifferentiationofCD34+cells3,4
- Isolationofendothelialprogenitorcells5
- Generationofhumanizedmice6
Columns
MS,LS,XS,orauto
MACS®Columns.