CpGODNsarewidelyusedforactivationofimmunecellsorinductionofsignallinginTLR9-expressingrecombinantcelllines.
ControlcompoundswhichcontainGpCdinucleotidesinsteadofCpGsareusefultocontrolfortheCpGspecificityofthiseffects.
TLR9isaprominentmemberofthetoll-like-receptor(TLR)familyrecognizingpathogen-associatedmolecularpatterns.TLR9recognizesspecificallyunmethylatedCpGmotifsinbacterialDNAle
ADIngtoactivationofimmunecells
1,2.Theseeffectscanbemimickedbyshortsyntheticaloligodeoxyribonucleotides(ODNs)containingunmethylatedCpGmotifs
3.
ControlcompoundsforCpGODNssharethesequencewiththeirstimulatorycounterpartsbutcontainGpCdinucleotidesinsteadoftheCpGmotifs.
ODN1826Control(ODN2138)canbeusedasasequencecontrolformurineB-classODN1826.