Components:- 200µglyophilizedODN
- 1mL1xTEBuffer
Overview
OligodeoxyribonucleotidescontainingCpGmotifs(CpGODNs)arewidelyusedforactivationofimmunecells,suchashumanPBMCs,murinesplenocytesorisolatedimmunecells,e.g.,BcellsandpDCs.TheyalsoactivatesignallinginTLR9-expressingrecombinantcelllines.
ODN1982isusedasacontrolforCpGODN,especiallyB-classCpGODNs.
Details
Backgroundinformation
TLR9isaprominentmemberofthetoll-like-receptor(TLR)familyrecognizingpathogen-associatedmolecularpatterns.TLR9recognizesspecificallyunmethylatedCpGmotifsinbacterialDNAle
ADIngtoactivationofimmunecells
1,2.Theseeffectscanbemimickedbyshortsyntheticaloligodeoxyribonucleotides(ODNs)containingunmethylatedCpGmotifs
3.
ControlcompoundsforCpGODNssharethesequencewiththeirstimulatorycounterpartsbutcontainGpCdinucleotidesinsteadoftheCpGmotifs.
Applications
ODN1982canbeusedasacontrolforCpGODN,especiallyB-classCpGODN.