MACSPlex Assays are designed for determining concentrations of soluble analytes in a single sample. The analysis is based on MACSPlex Capture Beads, which display defined fluorescence properties and can be identified using standard flow cytometry techniques.
MACSPlex Capture Beads within the MACSPlex Cytokine 12 Kit, human and MACSPlex Cytokine 10 Kit, mouse contain a cocktail of various fluorescently labeled bead populations, each coated with a specific antibody reacting with one of the respective cytokines within the sample.
In combination with the Express Modes of the MACSQuant
® Analyzers, the MACSPlex Cytokine Kits are optimized for automated measurement of beads. They simplify flow cytometric analysis via predefined experiment settings as well as acquisition and analysis templates. They apply an automated gating strategy to populations of interest that will be automatically adjusted for each data file individually to achieve optimal results.