Thestandardizeddissociationofneonatalmouseandratheartsisachievedbyacombinationofenzymaticandmechanicaldissociation.Afterdissociationthesampleisappliedtoafiltertoremovelargerparticlesandthecellsareimmediatelyreadyfordownstreamapplications.
TheNeonatalHeartDissociationKit,mouseandrat,hasbeenoptimizedfordownstreamcultivationofbeatingcardiomyocytes.However,awiderangeofcelltypescanbeobtainedforsubsequentcultivation,includingcardiacfibroblasts,cardiac
Progenitors,andendothelialcells.Additionaldownstreamapplicationsincludeenumerationandphenotypingofindividualcellpopulationsbyflowcytometryorfluorescencemicroscopy.