Clone105HB29recognizesthec-seriesganglioside–specificantigenA2B5.TheAnti-A2B5antibodybindstoglial
Progenitorsfromembryonictoadulthuman,murine,andrattissue.
A2B5ispredominantlyexpressedinembryonicandneonatalneuraltissue.Intheadultmammalianbrain,A2B5expressionisrestrictedmainlytoareaswhichretainneurogenicpotentialsuchasthesubventricularzone(SVZ).Thus,A2B5isconsideredasa
Markerforimmatureglial-committedprecursorswhicharepermanentlygeneratedintheSVZ.Glialprecursorcellsaredefinedascellsthatgiverisetoglialcelltypes,suchasastrocytesandoligodendrocytes.
GangliosideGT3anditsO-acetylatedderivativearetheprincipalA2B5-reactivegangliosides,andbothantigensaredown-regulatedasthecellsdifferentiateintomatureoligodendrocytes.Therefore,A2B5servesasamarkertomonitorthematurationofoligodendrocyteprogenitorsinoligodendrocytecultures.