TheCD309(Flk-1)MicroBeadKithasbeendevelopedfortheisolationofCD309(Flk-1)+cellsfromdifferentiatedmurineESoriPScellcultures.IsolationofCD309+cellsfromheterogeneousdifferentiationculturescanimprovedifferentiationintospecifichematopoieticorcardiovascularlineages,e.g.endothelialcells.
CD309isaclass3receptortyrosinekinase,alsoknownasFlk-1(fetalliverkinase1),vascularendothelialgrowthfactorreceptor2(VEGFR2),orkinaseinsertdomainreceptor(KDR).CD309isoneoftheearliestdifferentiation
Markersforendothelialandhematopoieticcells.
1,2UsingmouseembryonicstemcellsithasbeenshownthatCD309isamarkerformultipotentcardiovascular
Progenitorcellsthatgiverisetocardiomyocyte,endothelial,andvascularsmoothmusclecelllineages.
3,4