Capacity:for25preparationsOverview
TheMitochondriaExtractionKit-Tissueisdesignedtoaccelerateandsimplifytheisolationofmitochondriafromhumanandmousetissue.Thekithasbeendevelopedforusewiththegentle
MACSDissociators.Theresultingtissuehomogenatesprovidetheidealbasisforsubsequentmagneticlabelingandisolationofintact,functionalmitochondriaathighyield.
Details
Backgroundinformation
TheMitochondriaExtractionKit-Tissueallowsthepreparationofhomogenatesfromthemostcommontissuetypes,e.g.,muscle,heart,liver,brain,orkidney.ThekitworksincombinationwithgentleMACSDissociatorsandintegratestissuehomogenizationandcelllysisinasinglestep.ThetissuehomogenatescanbefurtherprocessedwiththeMitochondriaIsolationKit,human,orMitochondriaIsolationKit,mousetissue.
Downstreamapplications
IsolatedmitochondriacanbefurtheranalyzedbyWesternblots1,6orfunctionalassays,includingmeasurementofO2consumption,membranepotential,ATP4,5,7,orrespiratorycontrol4.IsolatedmitochondriaarewellsuitedformitochondrialRNAexpressionprofiling2,3.