Duringoligodendrocytedevelopment,lateA2B5
+oligodendrocyte
ProgenitorsstarttoexpressO4.Asthecellsdifferentiate,A2B5expressionceases,whereasO4expressionpersists.Morematurecellsstarttosynthesizegalactocerebroside,MBP,PLP,MOG,andO1.Intheperipheralnervoussystem,O4expressionisfoundonSchwanncells.DissociatedmousebraintissuederivedfromP3–7CD1micecontainsapproximately5–10%O4
+cells.
Forpositiveselection:MS,LS,orauto
MACS®Columns.Fordepletion:LDorautoMACSColumns.