Capacity:for100preparationsOverview
TheNeurosphereDissociationKitshavebeendevelopedforthegentleandefficientgenerationofsingle-cells
USPensionsfromculturedneurospheres.Inlessthananhour,thegentleenzymaticdissociationprocedureefficientlyyieldshighnumbersofviablecells.
Dissociatedcellsareimmediatelyreadyforfurther
invitroor
invivoapplicationssuchascellcultureandexpansion,flowcytometry,fluorescencemicroscopy,andmolecularapplications,orforcellseparationsusing
MACS®Technology.
Details
Backgroundinformation
Neurospheresarenon-adherentsphericalclustersofcellsformedwhenneuralstemcellsareculturedunderappropriateconditions.Effectivedissociationisimportantforeffectivepasagingandsubsequentdownstreamapplications
Someantigenepitopesaredegradedbypapain,othersbytrypsin.ThekitbasedonpapainisbestsuitedforusewithAnti-Prominin-1MicroBeads,CD11b(Microglia)MicroBeadsandAnti-A2B5MicroBeads,whereas,forexample,Anti-GLAST(ACSA-1)MicroBeadKitandAnti-PSA-NCAMMicroBeadsrequiretheuseofthetrypsin-basedkit.
PleaseseeFigure2forfurtherinformationwithregardtoepitopesensitivityandkitselection.