Forefficienttissuedissociation,theepidermalcelllayerisfirstremovedfromthedermalcelllayerafteranovernightenzymaticdigestion.Inasecondstep,theepidermallayerisenzymaticallydigestedandsubsequentlydissociatedintoasingle-cellsuspension,eithermanuallyorinanautomatedfashionbyusingthegentle
MACS™DissociatorandgentleMACSOctoDissociator.