Source:E.coli
Content:10µgOverview
Flt3-LigandstandsforFms-relatedtyrosinekinase3ligand.HumanFlt3-Ligandisarecombinantproteinoptimizedforuseincellculture,differentiationstudies,andfunctionalassays.
Applications
HumanFlt3-Ligandcanbeusedforavarietyofapplications,including:
- InvitroexpansionofCD34+hematopoieticProgenitorcells.
- DifferentiationofES-derivedcellstowardsthehematopoieticlineage.
- InvitrogenerationofLangerhanscells,dendriticcells,oreosinophilsfromCD34+cells.
Details
Backgroundinformation
Fms-relatedtyrosinkinase3ligand(Flt3-Ligand)isagrowthfactorthatregulatesearlyhematopoiesis.Flt3-Ligandbelongstoasmallfamilyofα-helicalcytokinesandpromotesinsynergywithothergrowthfactorslikeG-CSF,GM-CSF,SCF,andIL-3theproliferationanddifferentiationofprimitivehematopoieticstemcells.EarlyBcelllineagedifferentiationaswellasexpansionofmonocytesandimmaturedendriticcellsisstimulated.Flt3-LigandisexpressedbyTlymphocytesandbonemarrowstromalfibroblastsasamembrane-boundandasolubleisoform.BothisoformssignalthroughthetyrosinekinasereceptorFlt3/Flk-2,whichisrestrictedtocellsofhematopoieticorigin.HumanFlt3-Ligandisalsoactiveonmousecells.
ProliferationofOCI-AML5cells(
NIBSC96/532)
researchgrade:≥2×105U/mg
premiumgrade:≥5×105U/mg
Qualitydescription
Research-gradecytokinesaresuitableforawidevarietyofcellcultureapplications.Theyaresterile-filteredpriortolyophilization.Generally,endotoxinlevelsare<0.1ng/μg(<1EU/μg),andpuritiesare>95%.Thebiologicalactivityistestedinappropriatebioassays.
Premium-gradecytokinesoffertheconvenienceofhighandwell-definedbiologicalactivitiesandallowexactunitdosingfordemandingapplications.Thebiologicalactivityisdeterminedafterlyophilizationandreconstitution,andnormalizedtoWHO/NIBSCstandardswheneveravailable.Ingeneral,endotoxinlevelsare<0.01ng/μg(<0.1EU/μg),andpuritiesare>97%.Lot-specificcertificatesofanalysisareavailableonrequest(
MACStec@miltenyibiotec.de).