Source:E.coli
Content:100µgOverview
EGFstandsforepidermalgrowthfactor.HumanEGFisarecombinantproteinoptimizedforuseincellculture,differentiationstudies,andfunctionalassays.
Applications
HumanEGFcanbeusedforavarietyofapplications,including:
- Proliferationanddifferentiationofawidevarietyofcelltypesderivingfromectodermandmesoderm.
Details
Backgroundinformation
Epidermalgrowthfactor(EGF)istheprototypeofthelargefamilyofEGF-likeproteinswithacommonstructuralmotifcomprisingthreeintramoleculardisulfidebonds.EGFisproducedbyvariouscelltypeslikemammaryglandcells,gutepithelialcells,andcellsinthenervoussystemandthekidney.ProductionofEGFisinducedbytestosteroneandinhibitedbyestrogens.EGFstimulatestheproliferationanddifferentiationofmesenchymalcells,actsasamitogenforfibroblasts,epithelialandendothelialcells,andpromotescolonyformationofepidermalcells.
Proliferationof3T3cells
researchgrade:≥0.5×106IU/mg
premiumgrade:≥0.8×106IU/mg (typicalactivity:2×106IU/mg)
Qualitydescription
Research-gradecytokinesaresuitableforawidevarietyofcellcultureapplications.Theyaresterile-filteredpriortolyophilization.Generally,endotoxinlevelsare<0.1ng/μg(<1EU/μg),andpuritiesare>95%.Thebiologicalactivityistestedinappropriatebioassays.