EndotoxinRemovalBeadsrepresenttheidealtoolforfastandeasyremovalofendotoxinandDNAfrompurifiedproteinpreparations.
Preparationsofrecombinantproteinsareoftencontaminatedwithendotoxins(lipopolysaccharides,LPS)andDNAfromhostbacteria.BacterialendotoxinsandDNAshowstrong
BIOLOGicaleffectsinmanymammalsaswellasincellcultures.Ifpurifiedrecombinantproteinsareusedfor
invivoor
invitroassays,efficientremovalofendotoxinsandbacterialDNAisrequiredtoavoidnon-specificdetrimentaleffects.
ThecovalentlyboundpolycationicmoietyoftheEndotoxinRemovalBeadsshowsstrongbindingtobothendotoxinsandDNA.
EndotoxinorDNAcanberemovedby98%atinitialconcentrationsrangingfrom2000EU/mLto10,000EU/mLor2000to10,000ngofDNApermL,respectively.Endotoxinremovalisperformedusingthe
MACSiMAG™Separator.
Note:EndotoxinRemovalBeadsare
notsuitableforusewithMACS®ColumnsandMiniMACS™,MidiMACS™,VarioMACS™,SuperMACS™,autoMACS®,μMACS™,or
ThermoMACS™Separators.