ThekitconsistsofAnti-Biotin
MACSiBead™ParticlesandbiotinylatedantibodiesagainstmouseCD3εandCD28.Anti-BiotinMACSiBeadParticlesloadedwithbiotinylatedantibodiesareusedtomimicantigen-presentingcellsandactivaterestingTcellsinsingle-cells
USPensionsfromspleenorlymphnodesaswellaspurifiedTcells.Tcellexpansionisachievedbyculturingandreactivationatday6–8ofculture.