The EPC Enrichment and Enumeration Kit is designed for the enumeration of circulating EPCs (cEPCs) from peripheral blood, cord blood, or leukapheresis products as well as EPCs from bone marrow. To ensure reliable enumeration results, the EPCs are enriched during the procedure to increase the sensitivity of the flow cytometric analysis.
The kit contains all reagents for:
- red blood cell lysis,
- EPC enrichment,
- blocking of Fc receptor–mediated non-specific labeling of non-target cells,
- enumeration of EPCs based on expression of CD34, CD133, and CD309,
- exclusion of CD14+ cells,
- exclusion of dead cells,
- isotype controls.
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