ThekitissuitableforflowcytometricidentificationandenumerationofIL-2-producingCD154+CD4+Tcellsuponinvitrostimulation.OtherapplicationsincludeidentificationandenumerationofIL-2-producing,antigen-specificCD154+CD4+Tcellsuponrestimulationormonitoringofspecificityofantigen-specificTcelllines.
TheCD154/IL-2/CD4DetectionKitisdesignedfortheintracellulardetectionofIL-2productioninactivatedCD4
+Tcells.ItcontainsanantibodyrecognizingCD154–aspecific
MarkerforTcellactivation–andallreagentsnecessaryfortheimmunofluorescentdetectionofIL-2-producingactivatedCD4
+TcellsfromwholebloodorPBMCs.
IL-2israpidlysecretedbynaiveThelpercellsandbycertainsubsetsofmemoryTcellsuponactivation.ItpromotesgrowthanddifferentiationofTcellsandhaspleiotropiceffectsonmanyotherleukocytes.ThequantitativeanalysisofIL-2-producingcellscanprovideimportantinformationonthecourseofanimmuneresponse.