Capacity:for100tests(1)Overview
ThekitissuitableforflowcytometricidentificationandenumerationofIL-4-producingCD154+CD4+Tcellsuponinvitrostimulation.OtherapplicationsincludeidentificationandenumerationofIL-4-producing,antigen-specificCD154+CD4+Tcellsuponrestimulationormonitoringofspecificityofantigen-specificTcelllines.
Details
Backgroundinformation
TheCD154/IL-4/CD4DetectionKitisdesignedfortheintracellulardetectionofIL-4productioninactivatedCD4
+Tcells.ItcontainsanantibodyrecognizingCD154–aspecific
MarkerforTcellactivation–andallreagentsnecessaryfortheimmunofluorescentdetectionofIL-4-producingactivatedCD4
+TcellsfromwholebloodorPBMCs.
BycombiningthedetectionofcytokineproductionwithstainingofCD154andCD4,thesensitiveandspecificdetectionofactivatedantigen-specificCD4
+Tcellsiseasilyposs
IBLe.
IL-4ispredominantlysecretedbyactivatedCD4+andCD4+effectorandmemoryTcells,basophils,andmastcells.IL-4especiallyinducesandsupportshumoralresponses,e.g.,byitseffectsontheactivation,proliferation,andantibodyproductionofBcells.