ThekitissuitableforflowcytometricidentificationandenumerationofIFN-γ-producingCD154+CD4+Tcellsuponinvitrostimulation.OtherapplicationsincludeidentificationandenumerationofIFN-γ-producing,antigen-specificCD154+CD4+Tcellsuponrestimulationormonitoringofspecificityofantigen-specificTcelllines.
TheCD154/IFN-γ/CD4DetectionKitisdesignedfortheintracellulardetectionofIFN-γproductioninactivatedCD4
+Tcells.ItcontainsanantibodyrecognizingCD154–aspecific
MarkerforTcellactivation–andallreagentsnecessaryfortheimmunofluorescentdetectionofIFN-γ-producingactivatedCD4
+TcellsfromwholebloodorPBMCs.