Capacity:for100tests(1)Overview
ThekitissuitableforflowcytometricidentificationandenumerationofTNF-α-producingCD154+CD4+Tcellsuponinvitrostimulation.OtherapplicationsincludeidentificationandenumerationofTNF-α-producing,antigen-specificCD154+CD4+Tcellsuponrestimulationormonitoringofspecificityofantigen-specificTcelllines.
Details
Backgroundinformation
TheCD154/TNF-α/CD4DetectionKitisdesignedfortheintracellulardetectionofTNF-αproductioninactivatedCD4
+Tcells.ItcontainsanantibodyrecognizingCD154–aspecific
MarkerforTcellactivation–andallreagentsfortheimmunofluorescentdetectionofTNF-α-producingactivatedCD4
+TcellsfromwholebloodorPBMCs.
BycombiningthedetectionofcytokineproductionwithstainingofCD154andCD4,thesensitiveandspecificdetectionofactivatedantigen-specificCD4
+Tcellsiseasilyposs
IBLe.
TNF-αissecretedbyactivatedCD4+Tcells,monocytes,macrophages,NKcells,andneutrophils.TNF-αisamediatorofinflammatoryimmuneresponsesandisselectivelytoxicformanytransformedcells,especiallyincombinationwithIFN-γ.