ThekitissuitableforflowcytometricidentificationandenumerationofIL-5-producingCD154+CD4+Tcellsuponinvitrostimulation.OtherapplicationsincludeidentificationandenumerationofIL-5-producing,antigen-specificCD154+CD4+Tcellsuponrestimulationormonitoringofspecificityofantigen-specificTcelllines.
TheCD154/IL-5/CD4DetectionKitisdesignedfortheintracellulardetectionofIL-5productioninactivatedCD4
+Tcells.ItcontainsanantibodyrecognizingCD154–aspecific
MarkerforTcellactivation–andallreagentsfortheimmunofluorescentdetectionofIL-5-producingactivatedCD4
+TcellsfromwholebloodorPBMCs.