The KIR Typing Kit allows the detection of human killer cell immunoglobulin-like receptors (KIRs) on genomic DNA level or the analysis of KIR expression on mRNA level.
1-5 The presence or absence of KIR genes is analyzed by PCR using carefully designed sequence-specific primers (SSPs), enabling the detection of all known 15 human KIR genes plus two pseudo genes. Allel coverage: IPD KIR Sequence database release 2.1, March 2009 (http://www.ebi.ac.uk/ipd/)
Each well of the 96-well PCR plate contains a lyophilized enzyme mix, including Taq DNA Polymerase, as well as positive control primers. Resuspension Buffer is simply mixed with the template and dispensed into the wells of the PCR plate. PCR products are ready for immediate analysis by electrophoresis due to the integrated gel loading buffer.