Capacity:for1×109totalcellsOverview
TheAnti-CLAMicroBeadKitwasdevelopedforthepositiveselectionordepletionofhumanCLA+cellsfromPBMCs,lymphoidandnon-lymphoidtissueforphenotypicalandfunctionalcharacterization.
Details
Backgroundinformation
Thecutaneouslymphocyte-associatedantigen(CLA)isaninduc
IBLecarbohydratemodificationofP-selectinglycoproteinligand1(PSGL-1)andfunctionsasligandforE-selectin.CLAisaskin-homingreceptorandisfoundonaminorsubsetofhumanTcellsthatinfiltratetheskin.Thispost-translationalmodificationofPSGL-1isdiscussedtoserveasmechanismtoregulatehomingofCD4
+andCD8
+memory/effectorTcellsfromperipheralbloodtotheskin;animmuneresponsethatplaysanessentialroleduringmanyinflammatoryandcertainmalignantskindiseases.
Inperipheralblood,CLAisexpressedonskin-homingmemory/effectorTcells,onfewBcells,NKcells,andalmostallmonocytesanddendriticcells,includingLangerhanscellsintheskin.
TheAnti-CLAMicroBeadKitcanbeusedincombinationwith
MACS®CellIsolationKitsorMACSMultiSortKits,forexample,withtheCD8
+TCellIsolationKitfortheisolationofCD8
+CLA
+memory/effectorTcells.
Detailedseparationprocedure
Theisolationisperformedbyatwo-stepprocedure.TheCLA+cellsarefluorescentlyandindirectlymagneticallylabeledwithAnti-CLA-PEandAnti-PEMicroBeads.
Columns
Forpositiveselection:MS,LS,XS,orautoMACS®Columns.Fordepletion:LD,CS,D,orautoMACSColumns.