Capacity:for1×109totalcellsOverview
Anti-Melanoma(MCSP)MicroBeadsweredevelopedfor
- enrichmentofdisseminatedmelanomacellsfromperipheralblood1,bonemarrow,leukapheresisharvest,andlymphoidtissueofmelanomapatients–eveninearlydiseasestages2,3,
- isolationofmelanomacellsfromsingle-cellsUSPensionsofprimarytumortissueorprimaryskincellculture.
Anti-MelanomaMicroBeadscanbeusedincombinationwiththeInsideStainKit.Thisenablesthein-columnstainingofenrichedmelanomacellsforintracellularantigens,e.g.,Melan-A.Byperformingcombinedenrichmentandstaining,asensitivityofmelanomacelldetectionofonemelanomacellin2×107PBMCbackgroundcellscanbeachieved4.ForconvenientsubsequentimmunocytochemicaldetectiontheMelanomaEnrichmentandDetectionKitcanbeused.
Details
Backgroundinformation
Theantibodyclone9.2.27recognizesthemelanoma-associatedchondroitinsulfateproteoglycan(MCSP)antigen,alsoknownashighmolecularweightmelanoma-associatedantigen,orNG2.MCSPisexpressedonmelanomacellsbutnotoncarcinomacells,fibroblastoidcells,orcellsofhematopoieticorigin.
Downstreamapplications
CellsenrichedwithAnti-Melanoma(MCSP)MicroBeadscanbeanalyzedbyimmunocytochemistry,flowcytometry,ormolecular
BIOLOGymethods.Theisolatedcellscanalsobecultured.
Columns
MS,LS,orauto
MACS®Columns.