Arangeofapplicationsfromreversetranscription(RT)-PCRtomicroarrayanalysisand
CDNAlibrarygenerationrequirehigh-qualityRNA.UsingmRNAinsteadoftotalRNAasstartingmaterialcansignificantlyimprovethesensitivityandspecificityoftheresults.Thisisparticularlyimportant,whenthestartingmaterialislimitedorraretranscriptsareanalyzed.µMACSandMultiMACSmRNAIsolationKitsenableahighlysensitivemRNAisolation,fastereventhantr
ADItionaltotalRNAisolations.
Aftercelllysisandclearingofthecelllysate,themRNAismagneticallylabeledwithµMACSOligo(dT)MicroBeads.DuetothesmallsizeoftheMicroBeads,thehybridizationtothepoly(A)+tailofthemRNAmoleculesiscompletedwithinseconds.ThesampleisloadedontoaMACSColumnplacedinthemagneticfieldofaµMACSSeparator.Afterwashing,themagneticallylabeledmRNAisretainedonthecolumn.ByapplyingElutionBufferonthecolumn,puremRNAiselutedfromthecolumn.TheµMACSmRNAIsolationKitsaredesignedformanuallow-throughputmRNAisolationwhereastheMultiMACSmRNAIsolationKitsaredesignedforhigh-throughputapplicationsina96-wellformat.TheMultiMACSKitsareusedincombinationwiththeMultiMACSM96Separatorthatcanbeintegratedinaroboticsystemtoachieveafullyautomatedapproach.